Microorganism strain <i>Pediococcus acidilactici </i>tak 589 coccobest as an antimicrobial and antioxidant probiotic

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Solution Overview

Problem

There is a need for a Pediococcus acidilactici strain with specific properties suitable for use in human food, animal feed, and fermentation of plant matter, which is safe, effective in preventing bacterial infections and oxidative stress, and maintains biological activity under various conditions.

Innovation Solution

The Pediococcus acidilactici TAK 589 Coccobest strain, in lyophilized form, is used as a probiotic additive in food, feed, and fermentation starter, exhibiting antimicrobial and antioxidative properties, and is resistant to antibiotics, ensuring stability and safety.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If antibiotics are administered to prevent infections and stimulate growth in farm animals, then disease resistance and growth rate are improved, but antibiotic-resistant pathogens emerge and spread through the food chain and environment

Engineering Contradiction:

Improvedisease resistanceVSAvoidantibiotic-resistant pathogens

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent applies this principle by using bacteriocins produced by Pediococcus acidilactici TAK 589 Coccobest to combat pathogenic bacteria. The bacteriocins convert the harmful presence of pathogens into a beneficial situation where the pathogens are inhibited by the probiotic's natural antimicrobial substances, eliminating the need for antibiotics and preventing resistance development

Inventive Principle:

Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The patent uses Pediococcus acidilactici TAK 589 Coccobest as an intermediary organism that mediates between the host animal and pathogenic bacteria. The probiotic strain colonizes the gastrointestinal tract and acts as a protective barrier, producing bacteriocins that inhibit pathogens while stimulating the host's natural immunity, thus replacing antibiotics with a biological mediator

Inventive Principle:

Principle #24Intermediary (Mediator)

2Reliability

If probiotic strains are selected based on safety criteria excluding antibiotic-resistant strains, then safety is improved, but the range of available strains with desired functionality is reduced

Engineering Contradiction:

ImprovesafetyVSAvoidstrain selection flexibility

Core Design Contradiction:

ReliabilityVSAdaptability or versatility

Solution Approach 1:

The patent applies this principle by changing the selection parameters for probiotic strains from conventional criteria to include specific functional properties. Pediococcus acidilactici TAK 589 Coccobest is selected based on its bacteriocin production capability, antioxidative enzymes, and probiotic properties, rather than merely excluding antibiotic resistance. This parameter change expands the functional versatility of safe probiotic strains

Inventive Principle:

Principle #35Parameter changes

3Reliability

If probiotics are administered to promote colonization resistance and enhance immunity, then resistance to infections and stress is improved, but the complexity of ensuring viability through intestinal tract increases

Engineering Contradiction:

Improvecolonization resistanceVSAvoidviability preservation system

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The patent applies this principle by changing the environmental parameters to which the probiotic is exposed during administration. The lyophilized form protects the bacteria from moisture and temperature extremes during storage and handling, while the encapsulation or formulation systems protect them from gastric acidity and bile salts in the intestinal tract, ensuring viability without complex delivery mechanisms

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Pediococcus acidilactici TAK 589 Coccobest effectively inhibits pathogens like Listeria monocytogenes and reduces oxidative stress, maintaining biological activity under temperature and pH variations, making it suitable for gastrointestinal health and fermentation processes.

Implementation Method 1

Methodology Applied

Scientific EffectAntimicrobial activity:

Implementation Method 2

Methodology Applied

Scientific EffectAntioxidative activity: Oxidation

Implementation Method 3

The Pediococcus acidilactici TAK 589 Coccobest strain, in lyophilized form

Methodology Applied

Scientific EffectLyophilization: Freeze Drying

Data Source

PatentUS12491219B2Microorganism strain <i>Pediococcus acidilactici </i>tak 589 coccobest as an antimicrobial and antioxidant probiotic

Publication Date: 2025.12.09 BIOCC OU

AI summary

The microorganism strain Pediococcus acidilactici TAK 589 CoccobEst is a new antimicrobial and antioxidant probiotic, CoccobEst inhibits the growth of the enteropathogens L. monocytogenes, E. coli, S. enteritidis, S. typhimurium, S. sonnei, S. aureus, E. faecalis and C. sakazakii, but does not inhibit lactobacilli of natural intestinal microbiota. The strain's antimicrobial properties are enhanced by periplasmic peptidoglycan hydrolases with proteolytic activity. CoccobEst is used as a zootechnieai feed additive to prevent bacterial infections in the gastrointestinal tract of farm animals and pets and to prevent and reduce diarrhea. CoccobEst has highly active antioxidant defense system, increasing the redox activity of glutathione and reducing oxidative stress in cells. Therefore, CoccobEst is used as a food supplement or functional food for humans to prevent bacterial infections of the gastrointestinal tract and to prevent and reduce oxidative stress. In addition, CoccobEst is used as a starter culture in fermentation of vegetables.