Phenol-Modified ECM Hydrogel for In Vivo-Like Artificial Tissue
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Solution Overview
Problem
Existing artificial tissues lack the ability to effectively mimic in vivo microenvironments and promote cell proliferation, differentiation, and interaction between heterogeneous cells, limiting their functionality and applicability in drug delivery and regenerative therapy.
Innovation Solution
A phenol derivative-modified, tissue-derived extracellular matrix derivative is crosslinked to form a hydrogel, which exhibits adhesive properties and biodegradability, allowing for the formation of tissue structures that simulate in vivo microenvironments and support cell proliferation and drug delivery.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If conventional artificial tissues are used, then structural simplicity is maintained, but the ability to mimic in vivo microenvironments and promote cell proliferation is insufficient
Solution Approach 1:
The patent uses composite materials by combining phenol derivative-modified extracellular matrix derivatives with crosslinking agents to create a hydrogel system that mimics the complex in vivo microenvironment. The composite structure incorporates multiple ECM components (collagen, elastin, glycoprotein) modified with phenol derivatives, enabling enhanced cell-proliferating potential and compartmentalization of heterogeneous cells while maintaining biocompatibility and biodegradability.
Solution Approach 2:
The patent applies local quality by creating compartmentalized structures within the artificial tissue that provide different microenvironmental conditions for different cell types. The phenol derivative-modified ECM derivative forms localized adhesive regions that specifically promote cell attachment and proliferation in certain areas, while maintaining overall tissue structure integrity. This allows heterogeneous cells to be compartmentalized and interact in a controlled manner.
2Productivity
If simple cell aggregates are used, then ease of manufacture is maintained, but the ability to perform tissue functions through cell-proliferating potential is insufficient
Solution Approach 1:
The patent applies preliminary action by pre-modifying the extracellular matrix derivative with phenol derivatives before constructing the artificial tissue. This pre-modification introduces adhesive properties and cell-proliferating potential into the ECM scaffold beforehand, so that when cells are introduced, they can immediately attach and proliferate effectively. The crosslinking is also performed in advance to stabilize the hydrogel structure before cell seeding, reducing the complexity of the overall manufacturing process.
3Strength
If non-modified extracellular matrix is used, then biocompatibility is maintained, but adhesive properties and tissue adhesiveness are insufficient
Solution Approach 1:
The patent applies parameter changes by chemically modifying the extracellular matrix derivative with phenol derivatives, which changes the chemical parameters of the ECM to introduce adhesive properties. The phenol derivative modification alters the surface chemistry of the ECM, enabling strong adhesion to tissues and cells while maintaining the underlying biocompatible protein structure. The crosslinking process further modifies the physical parameters by creating a three-dimensional hydrogel network with enhanced mechanical strength and adhesiveness.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The hydrogel composition enhances cell adhesion, promotes hemostasis and blood coagulation, facilitates cell differentiation, and supports drug delivery, while being biocompatible and non-toxic, thus improving the functionality and applicability of artificial tissues in regenerative therapy.
Implementation Method 1
a phenol derivative-modified, tissue-derived extracellular matrix derivative is crosslinked to form a hydrogel
Implementation Method 2
a process (b) of crosslinking the modified, tissue-derived extracellular matrix to form a hydrogel
Data Source
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AI summary
The present invention relates to a phenol derivative-modified, tissue-derived extracellular matrix derivative for construction of artificial tissues. A hydrogel composition of the present invention and a hydrogel prepared therefrom exhibit various effects such as hemostasis, blood coagulation acceleration, cell transplantation, drug delivery, cell differentiation promotion, etc. and thus can be applied to single or composite uses. Furthermore, the present invention is excellent in biocompatibility due to its biodegradability and being almost free of cytotoxicity, thus exhibiting very high applicability. In addition, a tissue structure including a single type or multiple types of cells can be formed through the cell-proliferating potential and adhesion of the hydrogel of the present invention and can better simulate in-vivo microenvironments.