Plant Serine Protease Suppression for Stable Recombinant Proteins

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Solution Overview

Problem

Recombinant expression of animal proteins in plant cells is often hindered by proteolytic degradation due to the activity of serine proteases, which has not been effectively addressed by existing methods.

Innovation Solution

Genetically modify plant cells to reduce the activity of serine proteases with a specific motif (SSRGPX1LKPDX2X3APGX4SGTSMSCPHX5PX6WSPX7AX8X9SAX10MTT) and introduce exogenous nucleic acid molecules to encode proteins of interest, thereby preventing proteolysis.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If plant cells are used for recombinant expression of animal proteins, then production capability is improved, but proteolytic degradation increases

Engineering Contradiction:

Improverecombinant protein productionVSAvoidprotein stability

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

The patent extracts and removes the harmful serine protease activity from the plant cell system by identifying and eliminating proteases with the specific motif SSRGPX1LKPDX2X3APGX4SGTSMSCPHX5PX6WSPX7AX8X9SAX10MTT, thereby preventing proteolytic degradation of recombinant proteins while maintaining production capability

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent changes the proteolytic parameter profile of plant cells by reducing the activity of specific serine proteases through genetic modification or chemical inhibition, altering the cellular environment to be compatible with recombinant animal protein stability

Inventive Principle:

Principle #35Parameter changes

2Reliability

If serine protease activity is reduced in plant cells, then protein stability is improved, but proteolytic function is worsened

Engineering Contradiction:

Improverecombinant protein stabilityVSAvoidproteolytic degradation

Core Design Contradiction:

ReliabilityVSObject-generated harmful factors

Solution Approach 1:

The patent converts the harmful proteolytic activity into a beneficial situation by using the knowledge of serine protease mechanisms to develop specific inhibitors or genetic modifications that selectively block degradation of recombinant proteins while maintaining or enhancing overall protein production and quality

Inventive Principle:

Principle #22Blessing in disguise (Convert harm into benefit)

Data Source

PatentUS12553059B2Plant serine proteases

Publication Date: 2026.02.17 UNIV FUR BODENKULTUR WIEN

AI summary

The present invention relates to a genetically modified plant or plant cell derived from a wild-type plant or plant cell, said wild-type plant or plant cell producing at least one serine protease comprising the motif SSRGPX1LKPDX2X3APGX4SGTSMSCPHX5PX6WSPX7AX8X9SAX10MTT (SEQ ID No. 1), whereinX1 is a peptide consisting of 7 amino acid residues, X2 is I or L, X3 is T or M, X4 is a peptide consisting of 27 or 28 amino acid residues, X5 is a peptide consisting of 12 amino acid residues, X6 is T or E, X7 is S or A, X8 is V or I, X9 is K or R and X10 is I or M, wherein the proteolytic activity of the at least one serine protease in the genetically modified plant or plant cell is reduced compared to its activity in the wild-type plant or plant cell, wherein the genetically modified plant or plant cell comprises at least one exogenous nucleic acid molecule encoding for at least one protein or polypeptide of interest.