Hydrolases TRIZ Cases

Crosslinked biomolecular condensates immobilize enzymes without losing catalytic activity, while enabling easy separation and reuse in biocatalysis.

Bioorthogonal tagging of sgRNA enables timed CRISPR-Cas9 shutdown, limiting persistent activity and reducing off-target editing.

Muscle-specific rAAV delivery restores CAPN3 activity in LGMD2A while avoiding the cardiac toxicity seen with earlier promoters.

Specific amino acid substitutions in an Arthrobacter esterase improve (1R,3R)-methyl cyhalothrate hydrolysis for cleaner cyhalothric acid production.

Engineered microorganisms express amidases to turn acetamide from oxidized nylon waste into acetate without added substrate, reducing pollution.

Reducing non-essential endogenous proteins frees cell resources to raise recombinant polypeptide yield and quality while limiting misfolding.

A reverse transcriptase-endonuclease fusion enables site-specific insertion of longer genetic sequences with higher integration efficiency.

Direct ovarian medulla injection of CRISPR/Cas9 bypasses PGC handling to speed germline-edited chicken generation and reduce biosafety risk.

Targeting the 26 bp pAPN deletion, this CRISPR-Cas12a assay enables rapid, specific on-site detection of AE26-CAAS disease-resistant pigs.

Modified gRNA stem loops, triplexes, and pseudoknots improve Type V CRISPR binding, cleavage, and gene editing specificity.

Layered enzymes, substrates, buffer, and a pH dye enable rapid, sensitive surface detection of organophosphate and carbamate residues.

A single GPDA enzyme converts glucosamine directly to fructose and ammonia, removing multi-enzyme steps to save time, energy, and resources.

Targeted FAD2 insertions and deletions raise oleic acid in canola seed oil while lowering linoleic and linolenic acid.

An optogenetic recombinase cassette destroys male embryos in ovo, shifting hatch ratios toward females and avoiding post-hatch culling.

Engineered immune cells add metabolic polypeptides to sustain proliferation, cytotoxicity, and survival under low-glucose, acidic tumor conditions.

Microfluidic droplets speed screening of diverse plastic-degrading enzymes by detecting particle breakdown and selecting active variants for optimization.

Suppressing Sof1 alongside Cly1.a expands lodicules, opens flowering, and raises hybrid seed productivity in Triticeae.

A freeze-dried Diutina rugosa enzyme preparation replaces costly purified lipases, raising conversion while cutting effluents and energy use.

Engineered Bxb1 and PhiC31 integrases improve large-cargo genome insertion while reducing off-target edits and chromosomal rearrangements.

Organic sulfonic acids or sulfate esters suppress TRACP-5a interference, enabling more selective and accurate TRACP-5b measurement.

Engineered urethanases selectively cleave polyurethane urethane linkages, enabling complete breakdown into defined monomers under mild conditions.

Rapid MicroOrganoSphere formation from a single biopsy enables uniform 3D models and efficient CRISPR delivery for timely drug screening.

Tandem telomerase target sites, exonuclease cleanup, and chromatography improve LcDNA purity, fidelity, and scale-up stability under GMP.

A Y-linked dCas9 cassette removes Y gametes during spermatogenesis to produce female-only mammalian offspring without smaller litters or GMO progeny.

Opaque reaction wells block excitation light from the reaction volume, reducing photodamage while enabling accurate fluorescent nucleotide sequencing.

Using UBQ10-driven CRISPR and a viral replicon, this case boosts plant gene targeting efficiency without selectable markers.

Chemically synthesized aptamers inhibit multiple RNases and protect RNA where protein inhibitors are costly or fail at high temperatures.

RNAi constructs and CRISPR/Cas9 editing suppress Ara h allergens in peanuts, reducing seed allergen content for safer consumption.

Targeting PAD2 residues 341-357 with a peptide-based antibody improves inhibitory activity and helps suppress citrullination.

Protease-deleted B. subtilis with added oxidoreductases improves folding and secretion of disulfide-rich recombinant phosphatases.

CRISPR-Cas9 knockout of OsLEC1 promotes rice callus differentiation, improving transformation efficiency without complex treatments or heavy metals.

Tissue-specific recombinant cell lines convert cannabinoid and terpenoid activity into measurable GPCR calcium signals for potency and batch consistency.

Adapter-targeted dCas or dArgonaute binding normalizes NGS library concentrations across samples, reducing under- or over-sequencing.

Culturing blastocyst-derived single cells with LIF, GSK-3 inhibitor, and Activin A maintains human XEN cell morphology and marker expression.

Reactive center shape matching across hydrolases enables minimal-mutation grafting of multiple catalytic pockets into one stable enzyme.

Reducing AT1 gene expression improves plant tolerance to alkaline stress, avoiding slow and costly soil treatment for saline-alkali land.

Time-resolved fluorescence and mathematical fitting let CRISPR-Cas13 quantify multiple RNA targets with high sensitivity and throughput.

Enzymatic segment assembly, annealing, and ligation raise oligonucleotide yield and fidelity at scale without column chromatography.

Multiple large targeting vectors recombine in one step to improve homologous recombination for large genomic replacements at target loci.

A blocked guide RNA cascade enables non-specific in vivo delivery while activating therapeutic nucleic acids only in target cells to limit toxicity.

Paired guide RNAs and Cas9 nickase variants improve genome targeting specificity while reducing off-target cleavage, toxicity, and delivery burden.

Reducing motif-specific plant serine protease activity prevents recombinant protein degradation and preserves stability in engineered plant cells.

A single engineered strain combines dual metabolic pathways to produce 1,3-PDO and 3-HP from glucose or glycerol without mixed cultures.

Reducing PHS1 expression suppresses B-type starch granules, improving filtration and sedimentation while maintaining starch content.

Targeted DHS gene edits reduce senescence without foreign DNA, improving shelf life, stress tolerance, biomass, and yield.

Site-specific recombination brings homologous sequences together to excise target DNA efficiently without leaving residual sequences.

Proline-specific endoproteases cut haze-causing beer proteins while preserving foam stability and avoiding extra filtration.

A conserved sequence motif enables in silico discovery of halogenases with broader substrate scope for selective, lower-by-product halogenation.

CRISPR/Cas editing of the HAO1 gene enables durable suppression of oxalate production in PH1 while reducing repeat dosing needs.

Immobilized gRNA-Cas complexes detect labeled target nucleic acids without PCR, enabling rapid multiplex pathogen panel development.