Polarized Bio-Detection Pixel Layout for Fluorescence Crosstalk
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Solution Overview
Problem
Existing bio-detection devices face issues with crosstalk between neighboring wells due to reduced pixel array sizes, leading to inaccurate analytical results.
Innovation Solution
A bio-detection device with pixel units containing pairs of reflective sub-polarizing units having a 90° polarizing angle difference, combined with a bio-detection system and method that involves immobilizing bio-samples on reaction sites and detecting fluorescent signals from these units to reduce crosstalk and enable higher pixel array density.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If pixel array density is increased by reducing spacing or well pitch, then manufacturing cost decreases and throughput increases, but crosstalk between neighboring wells occurs and measurement precision deteriorates
Solution Approach 1:
The patent divides the pixel array into multiple sub-pixels, with each sub-pixel containing a reflective sub-polarizing unit. This segmentation allows independent optical control for each sub-pixel, enabling precise separation of fluorescent signals from neighboring wells while maintaining high pixel array density. The sub-pixel structure physically isolates the optical paths to eliminate crosstalk.
Solution Approach 2:
The patent applies different polarizing angles (0° and 90°) to different sub-pixels within the same pixel unit. This local differentiation creates distinct optical characteristics for adjacent sub-pixels, allowing the system to distinguish between neighboring wells based on their unique polarization signatures. The local quality variation enables precise signal identification without requiring physical separation.
2Device complexity
If pixel array size is reduced to increase pixel density, then device integration improves, but crosstalk between neighboring wells increases and analytical accuracy decreases
Solution Approach 1:
The patent segments each pixel into multiple sub-pixels with reflective sub-polarizing units, allowing high pixel density while maintaining optical isolation. The sub-pixel structure with its reflective elements creates distinct optical paths that prevent signal leakage between neighboring pixels, thus maintaining reliability even at high integration densities.
Solution Approach 2:
The reflective sub-polarizing units act as intermediary elements between adjacent pixels. These units with their specific polarizing angles (0° and 90°) serve as optical mediators that direct and separate fluorescent signals, preventing crosstalk while enabling close spacing of pixels. The intermediary structure allows high integration without sacrificing signal integrity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution effectively reduces crosstalk between fluorescent signals, allowing for accurate analytical results and higher pixel array density in bio-detection devices.
Implementation Method 1
one or more pairs of reflective sub-polarizing units... The difference of the absolute value between respective polarizing angles of the reflective sub-polarizing units in each pair of reflective sub-polarizing units is 90°
Implementation Method 2
reflective sub-polarizing units... The one or more pairs of reflective sub-polarizing units are disposed on the substrate
Implementation Method 3
The fluorescent molecules may be excited by an excitation light with a shorter wavelength and generate an emission light with a longer wavelength toward the photodetector
Data Source
AI summary
A bio-detection device is provided. The bio-detection device includes a plurality of pixel units. Each of the pixel units includes a substrate, one or more pairs of reflective sub-polarizing units, and a plurality of reaction sites. The pairs of reflective sub-polarizing units are disposed on the substrate. The difference of the absolute value between respective polarizing angles of the reflective sub-polarizing units in each pair of reflective sub-polarizing units is 90°. The reaction sites are defined above the one or more pairs of reflective sub-polarizing units. The reaction sites and the reflective sub-polarizing units are in one-to-one correspondence.