Polycrystalline Lateral Flow Strip for Multiplex SERS Detection
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Solution Overview
Problem
Conventional lateral flow assay strips face challenges in detecting low-concentration biological specimens with high sensitivity and simultaneously detecting multiple targets, and are difficult to form multi-detection lines and control detection line spacing, especially for heterogeneous materials.
Innovation Solution
A lateral flow assay strip with a polycrystalline structure formed by clusters of nanoparticles, incorporating a specimen area, test area with multiple detection parts, and absorption area, utilizing Surface Enhanced Raman Spectroscopy (SERS) or plasmon-enhanced fluorescence (PEF) analysis, and featuring vertical and horizontal detection lines with a hydrophobically treated area for enhanced signal detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional lateral flow assay strips are used, then the structure is simple and easy to manufacture, but the sensitivity for detecting low-concentration biological specimens is insufficient
Solution Approach 1:
The patent employs a composite structure combining polycrystalline material with multiple grain boundaries integrated into the lateral flow assay strip. This polycrystalline composite structure enhances signal intensity and detection sensitivity while maintaining compatibility with the existing strip architecture, thereby improving measurement precision without excessive increase in device complexity
Solution Approach 2:
The invention introduces detection lines with specific polycrystalline structures at localized positions within the test area of the strip. By concentrating the enhanced polycrystalline detection structures only where needed (at detection lines) rather than throughout the entire strip, the patent improves local detection sensitivity while minimizing overall structural complexity
2Manufacturing precision
If conventional lateral flow assay strips are used, then the manufacturing process is simple, but it is difficult to form multiple detection lines and control their spacing
Solution Approach 1:
The patent incorporates detection lines with predetermined polycrystalline structures during the manufacturing process. By pre-forming these structured detection lines with controlled spacing and positions before final assembly, the invention achieves precise control over detection line spacing and multi-line configuration, thereby improving manufacturing precision
Solution Approach 2:
The invention divides the test area into multiple distinct detection lines, each with specific polycrystalline structures. This segmentation allows independent optimization and control of each detection line's position and spacing, enabling precise multi-target detection while maintaining manageable manufacturing processes through modular design
3Adaptability or versatility
If conventional lateral flow assay strips are used, then the detection method is simple, but the ability to simultaneously detect multiple targets at high sensitivity is limited
Solution Approach 1:
The patent designs the lateral flow strip with multiple detection lines that can simultaneously detect different targets. Each detection line is equipped with polycrystalline structures that enhance sensitivity, allowing the single strip to perform multiple detection functions concurrently. This multi-functional design enables simultaneous detection of various analytes including cells, proteins, and nucleic acids with high sensitivity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The assay strip enables high-sensitivity on-site diagnosis of non-label biological specimens and simultaneous detection of various targets, with improved signal intensity and uniformity through the polycrystalline structure, facilitating precise detection of cells, proteins, nucleic acids, and other biological materials.
Implementation Method 1
utilizing Surface Enhanced Raman Spectroscopy (SERS) or plasmon-enhanced fluorescence (PEF) analysis
Implementation Method 2
utilizing Surface Enhanced Raman Spectroscopy (SERS) or plasmon-enhanced fluorescence (PEF) analysis
Implementation Method 3
a biochemical reaction occurs between a target included in a specimen and a probe as the specimen flows by a capillary action
Data Source
Figure 1~2(e)
Figure 3(a)~4(d)
Figure 5(a)~6
AI summary
The present disclosure provides a lateral flow assay strip having a polycrystalline structure and a preparation method thereof. More specifically, the present disclosure provides a lateral flow assay strip using a technique of growing a plasmonic polycrystalline structure and having a polycrystalline structure to allow on-stie diagnosis with high sensitivity and simultaneously detect a plurality of targets and an effective preparation method thereof.