Probiotic Composition for Reducing Gut Antibiotic Resistance

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Solution Overview

Problem

The rapid emergence and dissemination of antibiotic resistance in gastrointestinal microbiota, particularly in food-producing animals, poses a significant threat to public health due to the lack of effective markers to monitor antibiotic-GI tract interactions and the dynamic nature of GI microbiota, which is influenced by antibiotics and ingested microorganisms, leading to contamination and environmental AR gene pools.

Innovation Solution

The use of Lactobacillus crispatus WZ-12 and its derivatives as probiotics to modulate gut microbiota by inhibiting the growth of antibiotic-resistant bacteria, reducing their colonization, and improving gut health through feed compositions and ingestible food products.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If antibiotics are used to treat infections, then pathogenic bacteria are eliminated, but antibiotic-resistant bacteria emerge and proliferate in GI microbiota

Engineering Contradiction:

Improveinfection treatment efficacyVSAvoidantibiotic-resistant bacteria proliferation

Core Design Contradiction:

ReliabilityVSObject-generated harmful factors

Solution Approach 1:

Probiotic bacteria serve as intermediary organisms that compete with antibiotic-resistant bacteria for colonization resources in the GI tract. The probiotics modulate the microbiota environment to favor susceptible bacteria over resistant strains, thereby reducing the harmful effects of antibiotic resistance without compromising infection treatment efficacy

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent converts the harmful presence of antibiotic-resistant bacteria into a benefit by using probiotics to selectively suppress resistant strains while promoting susceptible ones. This transforms the post-antibiotic microbiota state from harmful (resistant dominance) to beneficial (susceptible dominance restored)

Inventive Principle:

Principle #22Blessing in disguise (Convert harm into benefit)

2Reliability

If probiotics are used to modulate gut microbiota, then colonization of pathogenic bacteria is hindered, but mechanisms of AR reduction by probiotics are not fully understood

Engineering Contradiction:

Improveprobiotic efficacyVSAvoidmechanism understanding

Core Design Contradiction:

ReliabilityVSLoss of information

Solution Approach 1:

The patent employs monitoring of microbiota composition and antibiotic resistance gene prevalence as feedback mechanisms to evaluate probiotic effectiveness. By tracking these parameters, the system provides information on how probiotics modulate the microbiota environment and suppress resistant bacteria, gradually filling the knowledge gap about reduction mechanisms

Inventive Principle:

Principle #23Feedback

3Reliability

If feed compositions containing probiotics are administered, then antibiotic-resistant bacteria colonization is reduced, but the dynamic nature of GI microbiota influenced by ingested microorganisms complicates monitoring

Engineering Contradiction:

ImproveAR bacteria colonization reductionVSAvoidmicrobiota composition monitoring

Core Design Contradiction:

ReliabilityVSDifficulty of detecting and measuring

Solution Approach 1:

The patent employs molecular markers and genetic sequencing techniques that detect specific DNA sequences associated with antibiotic resistance genes. These molecular 'signatures' allow precise identification and quantification of resistant bacteria amidst the dynamic microbiota, overcoming the complexity of monitoring by providing specific detectable targets

Inventive Principle:

Principle #32Color changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Lactobacillus crispatus WZ-12 effectively reduces the prevalence of antibiotic-resistant bacteria in the gastrointestinal tract, mitigating AR and enhancing gut health in animals, thereby addressing the challenge of antibiotic resistance in poultry production systems.

Implementation Method 1

The antimicrobial and microbiota-modulation activity of probiotics can hinder colonization of pathogenic bacteria

Methodology Applied

Scientific EffectProbiotic inhibition:

Data Source

PatentUS12558386B2Methods and compositions to modulate antibiotic resistance and gastrointestinal microbiota

Publication Date: 2026.02.24 OHIO STATE INNOVATION FOUND

AI summary

Disclosed herein are methods and compositions related to probiotic formulas. These formulas comprise the bacterial strain Lactobacillus crispatus WZ-12 or a derivative thereof. Other strains which can be included in the composition include, but are not limited to, Lactobacillus salivarius 1-14, or Lactobacillus reuteri 2-2, and derivatives thereof. These compositions can be used in a variety of methods to treat and/or ameliorate diseases, such as colonization of antibiotic-resistant bacteria in the host organism. Also disclosed herein are methods of treating those subjects in need thereof by administering the compositions disclosed herein.