Protease and Amylase Variants for Low-Temperature Detergent Stability
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Solution Overview
Problem
Current detergent compositions lack improved protease and alpha-amylase variants that offer enhanced stability and wash performance, particularly at low temperatures and in the presence of detergent ingredients like bleach and surfactants.
Innovation Solution
The development of detergent compositions incorporating protease variants with substitutions at specific positions, such as 171, 173, 175, 179, or 180, and alpha-amylase variants with specific amino acid sequences or modifications, which maintain at least 75% sequence identity to parent enzymes but exhibit improved stability and activity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional protease and amylase are used in detergent compositions, then the detergent can perform basic cleaning functions, but the enzyme stability and wash performance are insufficient, particularly at low temperatures and in the presence of bleach and surfactants
Solution Approach 1:
The patent applies parameter changes by modifying the amino acid sequence of protease and amylase enzymes through site-directed mutagenesis. Specific amino acid residues are substituted to alter enzyme properties, resulting in variants with improved stability at low temperatures and enhanced resistance to detergent ingredients like bleach and surfactants, while maintaining or improving catalytic activity
Solution Approach 2:
The patent applies local quality by making targeted modifications at specific positions within the enzyme structure rather than global changes. Site-directed mutagenesis introduces specific amino acid substitutions at predetermined residues, allowing localized optimization of enzyme stability and activity while preserving the overall structure and function of the parent enzyme
2Stability of the object's composition
If enzyme variants with improved stability are developed through amino acid substitutions, then detergent stability and storage stability are enhanced, but the complexity of enzyme development and characterization increases
Solution Approach 1:
The patent applies segmentation by dividing the enzyme development process into systematic stages: selecting parent enzymes, identifying target residues for substitution, performing site-directed mutagenesis, expressing variants in host cells, and characterizing enzymatic properties. This segmented approach manages complexity by breaking down the overall development process into manageable, sequential steps
3Productivity
If protease variants with substitutions at positions 171, 173, 175, 179, or 180 are created, then wash performance and detergent stability are improved, but the sequence identity to parent enzyme decreases below 100%
Solution Approach 1:
The patent applies parameter changes by intentionally modifying the amino acid sequence at specific positions (171, 173, 175, 179, or 180) to improve enzyme performance. The substitution of amino acid residues at these positions alters local structure and function, enhancing wash performance and detergent stability while accepting reduced sequence identity as a necessary trade-off for functional improvement
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
These variants demonstrate increased stability and improved wash performance compared to their parent enzymes, particularly in detergent formulations, even at low temperatures and in the presence of challenging detergent ingredients.
Implementation Method 1
The term 'protease' is defined herein as an enzyme that hydrolyses peptide bonds
Implementation Method 2
The term 'alpha-amylase' means an alpha-amylase having alpha-amylase activity, i.e. the activity of alpha-1,4-glucan-4-glucanohydrolases, E.C. 3.2.1.1, which constitute a group of enzymes, catalysing hydrolysis of starch and other linear and branched 1,4-glucosidic oligo- and polysaccharides
Data Source
AI summary
The present invention relates to detergent compositions comprising protease variants and alpha-amylases or variants thereof. Furthermore, the present invention relates to methods of using the detergent compositions.