Hydrolases TRIZ Cases

OMNI-103 CRISPR nuclease targets specific DNA sequences via guide RNA complexes, reducing off-target effects and improving in vivo applicability.

Randomized PAM sequences in a plasmid DNA library identify target sites, resolving low specificity and high costs associated with redesigning nucleases.

Maternal accumulation of three or more Cas9 gene copies overcomes guide RNA introduction limits, enabling simultaneous editing of multiple target genes.

Genome modification of microbial host cells reduces non-ribosomal peptide synthase production to enhance fermentation yield.

Hierarchical metal-organic frameworks immobilize enzymes in large channels while smaller channels enable reactant diffusion.

Replacing endogenous sequences with human TTR cDNA enables accurate pharmacokinetic testing of reagents targeting the V30M mutant protein.

Engineered Cas12a proteins with optimized amino acid sequences enhance genome editing efficiency through precise nucleic acid cleavage.

Enzymatic pathways introduce vinyl groups into precursors to synthesize isoprene without terminal alcohol phosphorylation.

Genetic deletion of PrtS, HtrA, and Ster-1612 proteases in Lactococcus lactis prevents protein degradation and increases heterologous yield.

Replacing Bst with Bsu LF or Klenow allows mesophilic Cas12 enzymes in a single step, avoiding high heat incompatibility.

Oligonucleotides facilitate DNA end joining to enhance chromosomal modification efficiency in plant cells.

Circular polynucleotide templates stabilize modification sequences in Escherichia coli cells to enable precise homologous recombination.

RuvC domain mutations in Cas9 reduce off-target DNA cleavage while maintaining on-target efficiency.

Engineered subtilisin variants with targeted amino acid substitutions enhance proteolytic activity in cleaning formulations.

Phospholipase and alpha-amylase treat crude oils to remove phosphorus without saponification, increasing yield.

Segmented guide and bait RNA structures resolve low precision in targeted transgene insertion by enhancing homologous recombination frequency.

Guide RNA directs Cas9 nickase to introduce single-strand breaks, enabling precise mapping of repetitive sequences and structural variants.

Mutating the crp gene reduces cAMP binding to CRP, lifting enzyme activity constraints and boosting biotin and thiamine production.

Site-directed mutagenesis creates enzyme variants that resist bleach degradation while sustaining wash performance at low temperatures.

Engineered cell lines with doxycycline-inducible human ceramidases enable precise inhibitor potency measurement.

Hepatic portal vein injection of sgRNA plasmids bypasses embryonic stem cell complexity to rapidly construct primate tumor models.

Segmenting FatB clades resolves specificity and efficiency trade-offs, enabling precise fatty acid enrichment in microalgae oils.

A nucleic acid sequence-recognizing module bonded to a deaminase enzyme converts targeted DNA bases in monocot cells without cleaving the double-stranded DNA.

PDE4D7 expression analysis replaces PSA testing to resolve false-positive rates and improve diagnostic reliability.

Modified Cas9 nucleases bind alternative PAM motifs to access previously untargetable genomic positions while reducing off-target effects.

Mutant thioesterases generate medium-chain fatty acids directly, eliminating unwanted by-products and reducing downstream separation costs.

Engineered aminoacyl tRNA synthetases incorporate non-standard amino acids into proteins.

Codon optimization of the Cas9 endonuclease improves expression efficiency in mammalian cells, resolving bottlenecks in versatile genomic modification.

CRISPR knockout of B2M and NKG2A combined with bispecific molecules prevents host NK cell rejection of allogeneic T cells.

RNA-guided gene drives use programmable guide RNAs to direct Cas9 nuclease activity toward specific DNA sequences.

A recombinant adeno-associated virus delivers a truncated ATP7B protein lacking metal-binding domains 1-3 to treat Wilson disease.

Modified Pantoea strains convert xylose to 2-ketoglutaric acid using xylonate dehydratase, eliminating glucose assimilation delays.

CRISPR editing attenuates toxin biosynthesis genes in aloe cell cultures for scalable bioreactor production.

ROK-1 thioesterase protein modifies the N-terminal region to suppress Oidium kalanchoeae infection, reducing reliance on fungicides.

A two-step genome editing method uses an intermediary CRISPR cut site to excise and replace genomic regions with guide RNAs.

A thermolabile DNase degrades contaminating double-stranded DNA in reaction mixtures before amplification.

DNA probes hybridize with abundant transcripts for targeted depletion, resolving reagent waste from high-abundance species.

Engineered hematopoietic stem cells with reduced CD123 expression repopulate the system after anti-CD123 therapy depletes native cells.

Sequential phospholipase and proteolytic enzyme treatments increase protein yield by at least 2% compared to traditional single-enzyme processes.

Nickase Cas9 variants paired with optimized guide RNAs reduce off-target effects while maintaining high editing efficiency.

A programmable transcription factor system links essential viability genes to specific promoter binding sites for precise genetic control.

TALEN nucleases target mutant dystrophin genes to enable precise sequence correction and functional protein restoration.

Stabilize single-stranded DNA donors using exonuclease-resistant modifications to resolve the trade-off between insertion efficiency and molecular stability.

CRISPR/Cas9 deletes Mypt1 exon 24 in vascular smooth muscle cells to enhance nitric oxide sensitivity.

CRISPR-mediated excision isolates complex genomic regions like CYP2D6, enabling accurate structural variant detection without amplification bias.