Subtilisin Variants for Enhanced Cleaning Performance
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Solution Overview
Problem
There is a need for engineered serine proteases that are suitable for specific conditions and uses, as existing serine proteases do not fully meet the requirements for particular applications.
Innovation Solution
Development of serine protease variants, specifically subtilisin variants with amino acid substitutions at specific positions, which are designed to enhance proteolytic activity and compatibility with various conditions, including the creation of cleaning compositions that utilize these variants.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If existing serine proteases are used, then basic proteolytic activity is provided, but cleaning performance and stability under specific conditions are insufficient
Solution Approach 1:
The patent applies parameter changes by modifying specific amino acid residues at defined positions in the subtilisin sequence (Lists 1-19) to optimize cleaning performance and stability. Multiple substitution options are provided at each position, allowing systematic variation of protein parameters to achieve desired performance characteristics under specific cleaning conditions.
Solution Approach 2:
The patent implements local quality by making targeted amino acid substitutions at specific positions rather than throughout the entire protein sequence. This localized modification approach allows optimization of specific functional regions while preserving the overall structure and basic proteolytic activity of the subtilisin enzyme.
2Productivity
If serine protease variants with amino acid substitutions are created, then proteolytic activity and cleaning performance are enhanced, but protein structure complexity increases
Solution Approach 1:
The patent systematically varies amino acid parameters at specific positions to enhance proteolytic activity. By providing defined substitution lists at each position, the patent creates a manageable framework for exploring sequence variations that improve productivity without random complexity.
Solution Approach 2:
The patent creates protease variants with multiple substitution options that can address different performance requirements. The same positional framework (Lists 1-19) can be used to generate variants optimized for different conditions, making the approach universally applicable across various cleaning applications while maintaining a consistent structural basis.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The serine protease variants exhibit improved cleaning performance and stability, offering enhanced proteolytic activity and adaptability for use in cleaning compositions, addressing the limitations of existing serine proteases.
Implementation Method 1
wherein said subtilisin variant is a mature form having proteolytic activity
Data Source
Figure 1
AI summary
The present invention provides serine protease variants, more specifically subtilisin variants produced there from. Specifically, the present invention provides serine protease variants, more specifically subtilisin variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants, more specifically subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants, more specifically subtilisin variants.