Yeast Cell Extraction Using Phospholipase and Proteolytic Enzymes
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Solution Overview
Problem
Current methods for extracting components from yeast cell cultures often result in low protein yields, making the production of yeast extracts economically inefficient.
Innovation Solution
Treating yeast cells with a purified phospholipase, either before, during, or after protein hydrolysis with proteolytic enzymes, to increase protein yield, specifically using phospholipase A1 or A2, and combining this treatment with proteolytic enzymes to enhance extraction efficiency.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional extraction methods are used, then the process is simple, but the protein yield is low
Solution Approach 1:
The extraction process is divided into multiple sequential treatment stages: initial phospholipase treatment to disrupt cell membranes, followed by proteolytic enzyme treatment for protein hydrolysis, and optional additional phospholipase treatment. This segmentation allows each enzyme to perform its specific function optimally, progressively breaking down cell structures to release proteins and achieving high protein yield (at least 2% increase) through cumulative effect rather than a single complex step.
Solution Approach 2:
Phospholipase treatment is applied as a preliminary action before or during proteolytic enzyme treatment. By first disrupting the cell membranes with phospholipase, the subsequent proteolytic enzymes can more effectively access and hydrolyze intracellular proteins. This preliminary membrane disruption action prepares the cell structures for more efficient protein extraction, resolving the contradiction between process simplicity and high protein yield.
2Productivity
If phospholipase treatment is added, then protein yield increases, but process complexity increases
Solution Approach 1:
The patent merges phospholipase treatment with proteolytic enzyme treatment into a unified extraction process. Rather than treating them as separate, independent steps, the method integrates them so that phospholipase prepares the cell structures and proteolytic enzymes simultaneously or sequentially act on the exposed proteins. This merging reduces the overall process complexity while maintaining the protein yield benefit of at least 2% increase compared to traditional single-enzyme methods.
3Productivity
If multiple enzymes are used, then extraction efficiency is enhanced, but manufacturing complexity increases
Solution Approach 1:
The method optimizes extraction efficiency by carefully controlling parameters such as enzyme concentrations, treatment times, temperatures, and pH levels for each enzyme stage. By adjusting these parameters, the process achieves high extraction efficiency with multiple enzymes (phospholipase and proteolytic enzymes) while keeping manufacturing complexity manageable through standardized parameter ranges that can be easily implemented in industrial settings.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method significantly increases protein yield by at least 2% compared to traditional extraction processes, improving the economic viability of yeast extract production by optimizing the separation and hydrolysis of yeast cell components.
Implementation Method 1
treating yeast cells with a purified phospholipase
Implementation Method 2
the phospholipase is selected from a phospholipase A1 or a phospholipase A2
Implementation Method 3
subjected to protein hydrolysis by a proteolytic enzyme
Implementation Method 4
proteolytic enzyme before, after, or during step i)
Implementation Method 5
separating the yeast extract from the treated yeast cells
Data Source
AI summary
The present invention relates to a method for extracting components from yeast cells with a purified phospholipase.