Protease Combination for Animal Feed Digestion
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Solution Overview
Problem
Current animal feed supplements lack a specific combination of neutral metalloprotease and serine alkaline protease activities, which are essential for optimizing protein digestibility and nutritional value, and often do not include amylase, limiting their effectiveness in improving amino acid availability for monogastric animals.
Innovation Solution
A protease combination comprising neutral metalloprotease and serine alkaline protease activities, derived from Bacillus amyloliquefaciens, is used in conjunction with amylase to enhance protein digestibility and nutritional value of animal feed, with specific activity ratios optimized for improved amino acid utilization.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If a single type of protease (e.g., serine protease only) is used in animal feed, then the feed supplement is simple to manufacture and cost-effective, but the protein digestibility and amino acid availability are insufficient
Solution Approach 1:
The patent combines multiple protease types (metallopeprotease and serine alkaline protease) with different catalytic mechanisms into a single feed supplement formulation. This merging allows the product to simultaneously utilize both metalloprotease activity for initial protein breakdown and serine alkaline protease activity for further peptide hydrolysis, thereby improving overall protein digestibility and amino acid availability without requiring separate supplements for each enzyme type.
Solution Approach 2:
The invention creates a composite enzymatic formulation containing both metalloprotease and serine alkaline protease activities. This composite approach leverages the complementary properties of different protease classes to achieve enhanced protein hydrolysis efficiency compared to single-enzyme preparations, while maintaining a unified product structure that is practical for feed supplementation.
2Device complexity
If protease supplements are used without amylase, then the formulation is simpler and less expensive, but the overall nutritional value and carbohydrate digestion are limited
Solution Approach 1:
The patent develops a multi-functional feed supplement that addresses both protein digestion (through dual protease activity) and carbohydrate digestion (through amylase inclusion). This universal formulation replaces the need for separate protease and amylase supplements, providing comprehensive digestive support in a single product while maintaining cost-effectiveness and ease of use.
3Quantity of substance
If protein-deficient diets are used to reduce cost, then feed expenditure is lower, but animal growth performance and amino acid availability deteriorate
Solution Approach 1:
The invention changes the enzymatic parameters of the feed supplement by incorporating specific ratios of metalloprotease and serine alkaline protease activities. This parameter optimization enables the enzymes to work synergistically at lower protein levels, maximizing the extraction of amino acids from available protein sources and maintaining growth performance even when dietary protein content is reduced.
Solution Approach 2:
The dual-protease formulation acts as an intermediary that facilitates more efficient protein utilization. By providing both metalloprotease and serine alkaline protease activities, the supplement mediates the breakdown of dietary protein into absorbable amino acids more effectively, allowing animals to maintain growth performance on protein-deficient diets that would otherwise be insufficient.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The protease combination significantly improves amino acid digestibility, growth performance, and feed conversion ratios in monogastric animals, even with protein-deficient diets, by facilitating better protein utilization and reducing undigested protein excretion, thus enhancing nutritional efficiency and sustainability.
Implementation Method 1
A protease, peptidase or proteinase, is an enzyme that catalyzes or increases the hydrolysis of proteins into smaller polypeptides or single amino acids
Implementation Method 2
The protease combination comprising neutral metalloprotease and serine alkaline protease activities, derived from Bacillus amyloliquefaciens, is used in conjunction with amylase to enhance protein digestibility
Data Source
AI summary
An enzyme composition containing a protease combination or a protease mixture having neutral metalloprotease and serine alkaline protease activity, and an amylase. Feed compositions, additives and formulations containing the enzyme composition in methods for improving digestibility of proteins in an animal diet or animal feed, as well as optimizing the nutritional value of an animal diet or animal feed.