Hydrolases TRIZ Cases

A mutant partial glyceride lipase catalyzes esterification of free fatty acids in polyunsaturated oils using short-chain monohydric alcohols.

An S582N substitution in the CLV1 receptor kinase increases locule number and seed production without compromising genetic stability.

A urine-based protein biomarker panel detects bladder cancer with high sensitivity and specificity.

Recombinant microorganisms express ergot alkaloid synthesis genes to produce ergolines, overcoming low yields of chemical methods.

A nucleic acid detection apparatus distributes samples into independent compartments for direct signal identification.

Anti-CRISPR ribonucleotides inhibit Cas-effector activity by preventing target nucleotide association.

Thioesterase expression modifies soybean oil fatty acid profiles, reducing linolenic acid content to improve oxidative stability for cooking applications.

PRIME-Del employs dual pegRNAs to nick opposite DNA strands, enabling precise deletion of sequences up to 10 kb without double-strand breaks.

A type IIs restriction enzyme assembles custom DNA vectors from molecular bricks in a single reaction step.

Engineered lipolytic enzyme variants modify flour lipids to enhance baked product quality.

Machine learning identifies design rules for single degenerate crRNAs that detect phylogenetically diverse targets, eliminating multiplexing complexity.

Cas9 proteins inhibit non-target amplification during isothermal reactions.

Engineered AAV capsids target central nervous system tissues with high efficiency, reducing liver tropism and immunological barriers.

SERCA inhibitors traffic misfolded hCLRN1N48K protein to inner ear hair bundles via ER stress induction.

Cas9 complexes protect target DNA segments from exonuclease digestion, isolating rare mutations amid abundant normal background.

CrisprZyme eliminates preamplification steps by coupling Cas13a reporter cleavage with nanozyme signal generation, enabling quantitative microRNA diagnostics.

Optimized linker lengths resolve the trade-off between packaging constraints and enzymatic cleavage rates.

Affinity chromatography and dialysis isolate native ubiquitin, resolving contamination by acid-resistant proteins while preserving structural integrity.

Diverting genes compete with product pathways for cofactors, channeling carbon flow to increase yields while maintaining cell growth.

Positioning specific residues at protease position 99 prevents lipase inactivation, maintaining storage stability and cleaning efficiency.

Rigid linker systems prevent misfolding and low yield in multi-functional chimeric proteins while maintaining enzymatic bioactivity.

Feruloyl esterase replaces harsh chemical hydrolysis, preserving plant components while generating pharmaceutical-grade trans-ferulic acid.

Mismatch guide RNA prevents target recognition, resolving low editing yields caused by CRISPR mismatch tolerance.

A CRISPR-Cas transposase biosensor detects nucleic acids using enzymatic FET strips and electronic multiplexors.

Fc binding peptide positions photoreactive groups to resolve non-specific reactivity and improve conjugation efficiency.

Mutagenesis of Rhizopus oryzae lipase enhances thermostability, enabling structured triacylglycerol synthesis above 60°C.

RNAi vectors silence TGD proteins while heterologous transcription factors drive lipid accumulation in plant leaves.

A photo-controlled CRISPR-Cas detection kit uses a silent guide RNA with a PC linker to activate Cas proteins via UV light.

Modified cutinase sequences enhance stability to remove sebum stains in detergents.

Custom peptide sequences paired with fluorophores resolve measurement precision issues in islet isolation by quantifying collagenase activity.

Glycosyl hydrolase enzymes expand cotton pore structures to trap encapsulated benefit agents, reducing costly substance loss during laundering cycles.

Guide RNA and Cas9 protein localize to target nucleic acids, resolving insufficient specificity in identifying sequences within complex mixtures.

Mutated GcoAB enzyme expands substrate range to include syringol, overcoming native enzyme limitations in lignin valorization.

Insertional enzyme complexes with tagmentation adapters produce gRNA libraries targeting non-coding genome regions without prior sequence knowledge.

Engineered LAGLIDADG nucleases target specific 22 basepair sites in maize, reducing off-target toxicity while improving recombination efficiency.

Biomaterial encapsulation stabilizes triazine hydrolase against environmental stressors, enabling efficient atrazine degradation without enzyme loss.

Specific amino acid mutations in the XylR regulator overcome diauxic growth inhibition to boost fatty acid derivative yields from mixed sugar feedstocks.

Neutral metalloprotease and serine alkaline protease activities improve amino acid availability despite low dietary protein levels.

Engineered retrons enhance multicopy single-stranded DNA production through modular genetic construct design.

CRISPR-Cas9 knockout of inhibitory genes in tumor infiltrating lymphocytes overcomes microenvironment suppression to boost persistence.

Fusion proteins combine enzymes with Cry protein domains to form stable crystals, enabling reuse in harsh industrial solvents and high temperatures.

Transplantable human fibrolamellar hepatocellular carcinoma tumor lines resolve ineffective chemotherapy by enabling reliable in vitro drug screening.

A recombinant Escherichia coli strain overexpressing acid phosphatase converts ascorbic acid into ascorbic acid-2-phosphate.

Precise HBB locus integration restores alpha globin balance, eliminating immune rejection risks from allogeneic transplants.