PTS EIIC A281V Variant for 5'-Inosinic Acid Production
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Solution Overview
Problem
Existing methods for producing 5'-inosinic acid using microorganisms are inefficient, and there is a need for improved strategies to enhance the productivity of this nucleic acid-based seasoning by targeting specific proteins involved in its biosynthetic pathway.
Innovation Solution
A novel PTS transporter subunit EIIC variant is developed by substituting alanine at position 281 with valine in the amino acid sequence, which is encoded by a specific nucleotide sequence, and introduced into a recombinant microorganism to increase the production of 5'-inosinic acid.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional microorganism culture methods are used for 5'-inosinic acid production, then the production process is simple, but the productivity is low
Solution Approach 1:
The invention applies parameter changes by modifying a specific amino acid residue (alanine to valine substitution at position 281) in the PTS transporter subunit EIIC protein sequence. This single amino acid substitution optimizes the transporter's function, thereby enhancing 5'-inosinic acid production efficiency without requiring complex multi-gene modifications or extensive strain engineering
2Productivity
If multiple proteins in the biosynthetic pathway are targeted for modification, then the potential for increasing productivity is higher, but the research time and complexity increase significantly
Solution Approach 1:
The invention applies local quality by focusing modifications on a specific critical component (PTS transporter subunit EIIC) rather than attempting to optimize multiple proteins throughout the biosynthetic pathway. The targeted amino acid substitution at position 281 locally optimizes sugar phosphate transport function, which is a rate-limiting step, thereby achieving productivity improvement without the time-consuming research required for comprehensive pathway optimization
3Productivity
If genetic recombination technology is applied to develop recombinant strains, then the production efficiency can be improved, but the manufacturing complexity increases
Solution Approach 1:
The invention applies parameter changes through a straightforward amino acid substitution (alanine to valine at position 281) in the EIIC protein sequence. This simple parameter modification can be implemented using standard molecular biology techniques without requiring complex multi-step genetic recombination procedures, thereby improving productivity while maintaining ease of manufacture
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The variant enhances the production of 5'-inosinic acid by up to 18% compared to the parent strain, demonstrating improved efficiency in producing this savory seasoning.
Implementation Method 1
a component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active transport system, and catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane
Data Source
AI summary
The present invention relates to a novel PTS transporter subunit EIIC variant and a method of producing 5′-inosinic acid using the same. The PTS transporter subunit EIIC variant is obtained by substituting one or more amino acids in the amino acid sequence constituting PTS transporter subunit EIIC to change the activity of the protein, and a recombinant microorganism comprising the PTS transporter subunit EIIC variant is capable of efficiently producing 5′-inosinic acid.