Transferases TRIZ Cases

Binding trans-prenyltransferase to a lipid membrane enables scalable enzymatic production of high-molecular-weight trans-polyisoprenoid for tires.

By loosening outer membrane-cell wall binding or adding channel proteins, cyanobacteria transfer electrons more efficiently across the cell boundary.

Specific motif A changes in Pyrococcus abyssi polymerase improve modified nucleotide incorporation while limiting sequencing misincorporation errors.

Osmolytes such as betaine enable elevated-temperature in vitro transcription that lowers dsRNA impurities and immunogenicity without sacrificing RNA yield.

Separating fermentation from enzymatic decarboxylation raises isobutene off-gas concentration, easing purification and reducing oxygen-related safety concerns.

Targeted mutations in 3-methyl-2-oxobutanoate hydroxymethyltransferase raise microbial pantothenic and pantoic acid production while preserving D-form purity.

By expressing Sfp and bpsA in flower tissues, this case creates stable, non-fading blue color from glutamine without vacuole pH limits.

Genetically engineered Auxenochlorella shifts heterotrophic oil production toward squalene, carotenoids, and omega-3 lipids with better sustainability.

Targeted amino acid substitutions tune alpha-1,3 branching activity, enabling glucan products with controlled and application-specific structures.

Blood-based ALDOC and BLBP assays enable objective TBI and SCI detection and repeated monitoring without subjective scoring or imaging.

Site-specific alaT promoter mutations raise aminotransferase activity to boost high-concentration L-alanine production in host cells.

An enzyme cascade using aminotransferase, aldehyde dehydrogenase, and oxidase enables mild, selective AMCCA production with lower energy use.

A D-ASFV pol X mirror-image polymerase enables chiral-specific L-DNA replication and L-RNA transcription for mirror nucleic acid drug screening.

Synthetic polymer extraction and pH-switched elution preserve membrane protein complexes, then remove the polymer for intact purification.

G-quadruplex RNA concatemers self-assemble into stable hydrogels without external crosslinkers, improving yield and protein expression.

Specific T7 RNA polymerase mutations raise co-transcriptional mRNA capping to 91-100%, cutting cap analog waste and purification cost.

An enzyme sequence excises, cleaves, and dephosphorylates a linking nucleotide to restore nucleic acid initiators for reuse and lower synthesis cost.

Chimeric TdT enzymes combine sequences from different species to improve 3'-O-blocked nucleotide incorporation and sequence uniformity.

Targeted amino acid substitutions tune alpha-1,3 branching activity, enabling glucan products with controlled branching patterns and defined structure.

Periodic 100-300 mg antibody dosing every 2-4 weeks keeps plasma kallikrein inhibition above 80 nM to control HAE without dose-limiting toxicity.

Engineered glucosyltransferase mutations convert rebaudioside D to rebaudioside M at high yield, improving biocatalytic production efficiency.

Engineered recombinant hosts express UGT enzymes to produce cleaner, more consistent steviol glycosides enriched in rebaudioside D.

Targeted substitution at AHAS subunit position 154 boosts Corynebacterium L-valine biosynthesis for more feasible industrial-scale production.

Poly(A) and poly(U) polymerases improve incorporation of 3′-O-blocked nucleotides for template-free synthesis of predetermined RNA and DNA sequences.

Thermolabile exonuclease and polymerase enable multi-fragment DNA assembly at room temperature, avoiding heating and manual handling steps.

Engineered cell lines express growth factor ligands and receptors internally, sustaining proliferation and higher culture density without exogenous supplements.

Engineered transaminases replace asymmetric hydrogenation to raise sitagliptin enantiomeric excess and yield in API manufacturing.

Overexpressing the soybean GmAK-HSDH gene raises water-soluble seed protein, improving soy processing quality and nutritional value.

Rationally mutated SB transposase improves solubility and stability for direct protein delivery, enabling controlled gene integration with rapid clearance.

Deleting ERG5 and ERG6 and adding codon-optimized DHCR24 and DHCR7 boosts cholesterol and precursor yields while limiting by-products.

Plasmid-encoded miRNA raises endogenous miRNA levels to degrade target mRNA, suppress kinase expression, and help restore homeostasis.

A Val179 substitution in rice AHASL1 enables spray-over tolerance to imidazolinone and sulfonylurea herbicides for more effective weed control.

Non-polar cyclic peptide residues improve cell uptake and cytotoxicity, enabling selective cancer cell killing at lower concentrations.

Engineered TdT variants enable template-independent DNA synthesis with removable 3′-blocked nucleotides, improving sequence control and length.

Targeted SDPS mutations let crops tolerate SDPS-inhibiting herbicides, expanding selective weed control and resistance management.

Recombinant plasmids upregulate miRNA that degrades target mRNA, lowering FLT3 bioavailability to help restore kinase-driven homeostasis.

A conserved-sequence acyltransferase expands dipeptide synthesis beyond narrow enzyme substrate limits, enabling 200+ products with green scale-up.

Recombinant lentiviral delivery at the tumor site inhibits the mevalonate pathway and activates gamma-delta T cells to destroy resistant cancer cells.

Plasmid-expressed miRNA sequences target kinase mRNA to lower kinase bioavailability, restore homeostasis, and support disease treatment.

An ATP regeneration loop converts both ADP and pyrophosphoric acid back to ATP, raising PAPS purity and conversion while cutting waste.

Boosting Zwf and CscK activity in Corynebacterium improves L-amino acid yield by redirecting metabolic flux with targeted genetic changes.

Engineered transaminases replace complex chiral synthesis routes, delivering high ee tryptamine derivatives with better solvent and thermal stability.

Targeted ilvN amino acid substitutions boost L-valine yield in Corynebacterium while avoiding broad pathway changes.

By converting PPO with transaminase and removing alpha-ketoacid by-products, this case raises L-glufosinate yield and eases isolation.

Specific MMLV RTase mutations raise thermostability and lower RNase H activity, improving long cDNA yield and fidelity from structured RNAs.

Covalent tagging and denaturing washes separate true in vivo RNA-protein interactions from spurious solution-phase binding.

Reporter-guided uORF mutations tune GGP expression to raise plant ascorbate while avoiding stunting, yield drag, and stress sensitivity.

CRISPR-edited CD38 epitopes in HSPCs and T cells reduce daratumumab binding, preserving healthy cells during anti-CD38 cancer therapy.

By recruiting endogenous GSK3 as a protease, this PROTAC approach enables rapid, specific degradation of target proteins.

A heterologous enzymatic route builds the QS-7 branched hexasaccharide, replacing plant purification with scalable precursor production.

A G746D (p)ppGpp synthetase variant boosts microbial 5'-inosinic acid production while avoiding broad pathway screening and long R&D cycles.

Targeted C-terminal truncation and a position 77 alanine substitution boost O-phosphoserine sulfhydrylase activity and L-cysteine yield.

Multi-site AGAT mutation and reaction tuning raise guanidinoacetic acid yield and conversion, enabling cleaner industrial production.

An engineered E. coli strain with the C24MTgm-M11 mutant boosts 3-aminoisobutyric acid fermentation yield while avoiding pollution from chemical synthesis.

Targeted A281V substitution in PTS transporter EIIC improves recombinant microorganism productivity without broad pathway modification.

This case uses GalNAc-conjugated siRNA to downregulate SMYD2, reducing liver fat and fibrosis in NAFLD and NASH.

Engineered SP6 RNA polymerase eliminates abortive transcription cycles to produce 80% full-length mRNA for therapeutic use.

Engineered recombinant DNA polymerases incorporate 3′-esterified nucleotide analogues through specific amino acid mutations.

Deleting specific enzymes like transaldolase redirects metabolic flux toward sedoheptulose accumulation, resolving low productivity in bacterial strains.

Recombinant host cells express aryl sulfotransferase to synthesize sulfated phenolic compounds.

PKR inhibitors stabilize transfected RNA, preventing degradation and sustaining expression levels.

Mutated Heliothis transposases overcome host inactivation to achieve high-frequency transposition and stable gene expression.