SDPS Herbicide Tolerance in Crops Through Targeted Enzyme Variants
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Solution Overview
Problem
Current herbicide tolerance technologies do not effectively target Solanesyl Diphosphate Synthase (SDPS) inhibiting herbicides, limiting the application flexibility and resistance management options for crop protection.
Innovation Solution
Development of transgenic plants overexpressing or editing the Solanesyl Diphosphate Synthase gene to confer tolerance to SDPS-inhibiting herbicides, using recombinant DNA technology and DNA editing techniques to introduce specific mutations or variants in the SDPS enzyme, combined with additional herbicide tolerance enzymes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If transgenic plants overexpressing or editing the Solanesyl Diphosphate Synthase gene are developed, then tolerance to SDPS-inhibiting herbicides is conferred, but the complexity of plant modification increases
Solution Approach 1:
The patent applies parameter changes by introducing specific amino acid substitutions (e.g., F240L, F227L, F229L, F247L) in the SDPS enzyme to alter its herbicide binding properties. These parameter changes at the molecular level confer tolerance to SDPS-inhibiting herbicides while maintaining enzyme function, resolving the contradiction between achieving reliability (herbicide tolerance) and avoiding excessive complexity.
2Adaptability or versatility
If additional herbicide tolerance enzymes are combined with SDPS variants, then resistance management options are enhanced, but the device complexity increases
Solution Approach 1:
The patent merges multiple functional elements into a single genetic construct: a modified SDPS enzyme with herbicide tolerance, coupled with a selectable marker gene (such as bar or pat) for herbicide resistance. This combining approach enhances adaptability and resistance management options while consolidating complexity into an integrated system rather than separate components.
Solution Approach 2:
The patent creates multi-functional genetic constructs where the SDPS variant provides both catalytic function (solanesyl diphosphate synthesis) and herbicide tolerance, while the associated selectable marker provides both selection capability and additional herbicide resistance. This multi-functionality enhances versatility without proportionally increasing system complexity.
Data Source
AI summary
The present disclosure relates to, inter alia, methods and compositions for weed control, for example, a method of selectively controlling weeds at a locus comprising crop plants and weeds by applying to the locus a weed controlling amount of a pesticide composition comprising a SDPS-inhibiting herbicide, wherein the crop plants are modified such that they comprise a SDPS which provides the crop plant with tolerance against the SPDS-inhibiting herbicide. Compositions also include, inter alia, recombinant polynucleotides suitable for use in the methods.