Pyripyropene Biosynthetic Gene Cluster Isolation and Expression

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Solution Overview

Problem

The industrial production of pyripyropenes is hindered by low productivity due to the limited amount of secondary metabolites produced by naturally occurring microorganisms, and there is a lack of reports on the isolation of their biosynthetic gene cluster, making it difficult to introduce and enhance the biosynthetic gene cluster in pyripyropene-producing microorganisms for improved productivity.

Innovation Solution

A nucleic acid construct comprising a pyripyropene biosynthetic gene cluster and a marker gene is expressed in a host, allowing for the significant improvement of pyripyropene productivity through genetic transformation, specifically by introducing the pyripyropene biosynthetic gene cluster into a suitable microorganism, such as Penicillium coprobium, to enhance pyripyropene production.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If naturally occurring microorganisms are used for pyripyropene production, then the production process is simple, but the productivity is low

Engineering Contradiction:

Improvepyripyropene production amountVSAvoidgene cluster isolation and introduction complexity

Core Design Contradiction:

ProductivityVSDevice complexity

Solution Approach 1:

The patent extracts the pyripyropene biosynthetic gene cluster from the natural microorganism genome and isolates it as a separate functional unit. This extracted gene cluster can then be introduced into host microorganisms to enhance pyripyropene production, resolving the contradiction between simple cultivation and low productivity

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent uses a vector as an intermediary carrier to transport the isolated pyripyropene biosynthetic gene cluster into host microorganisms. This mediator enables the transfer of genetic material, allowing productivity improvement without requiring direct manipulation of the host genome

Inventive Principle:

Principle #24Intermediary (Mediator)

2Productivity

If biosynthetic gene expression is enhanced through gene recombination, then productivity improves, but the difficulty of gene isolation and transformation increases

Engineering Contradiction:

Improvepyripyropene production amountVSAvoidgene isolation and transformation ease

Core Design Contradiction:

ProductivityVSEase of manufacture

Solution Approach 1:

The patent successfully extracts and isolates the pyripyropene biosynthetic gene cluster from natural microorganisms, making it available for genetic manipulation. This extraction enables subsequent ease of manufacture through standardized transformation protocols

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent develops a transformation method that can be applied to multiple host microorganisms, making the gene introduction process universally applicable. This multi-functionality approach standardizes the transformation procedure, improving ease of manufacture across different experimental systems

Inventive Principle:

Principle #6Universality (Multi-functionality)

Data Source

PatentEP2530149B1Nucleic acid structure containing a pyripyropene biosynthesis gene cluster and a marker gene

Publication Date: 2017.10.25 MEIJI SEIKA KAISHA LTD

AI summary

There is provided a nucleic acid construct comprising a pyripyropene biosynthetic gene cluster and a marker gene. The nucleic acid construct according to the present invention provides an inexpensive and highly productive method for producing pyripyropene.