Rapeseed Protein Isolate Extraction Without Fractionation
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Solution Overview
Problem
Existing methods for obtaining highly soluble rapeseed protein isolates often require additional fractionation steps, resulting in protein wastage and reduced quality, as they typically separate the 7S and/or 12S fractions, which are not suitable for human nutrition.
Innovation Solution
A process involving cold-pressed rapeseed meal extraction without separating protein constituents, using an aqueous salt solution at 45-65°C, followed by decantation, pH adjustment, and precipitation with divalent ion salts, then concentrating and washing to produce a native rapeseed protein isolate comprising both cruciferins and napins, maintaining high solubility across a broader pH range without fractionation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If additional fractionation steps are used to obtain highly soluble rapeseed protein isolates, then solubility is improved, but protein wastage increases and quality is reduced
Solution Approach 1:
The patent changes the extraction parameters by using a buffered aqueous solution at pH 7.0 and temperature of 45-65°C, which optimizes the solubility of both cruciferins and napins simultaneously. This parameter optimization allows highly soluble protein isolate (≥88% solubility) to be obtained without fractionation, thus preventing protein wastage while achieving the desired solubility.
2Reliability
If fractionation steps separate 7S and/or 12S fractions, then solubility is improved, but protein quality is reduced
Solution Approach 1:
The patent merges the extraction of both cruciferins (12S fraction) and napins (7S/2S fractions) into a single extraction step using buffered aqueous solution. This combined extraction preserves the complete protein profile and functional quality of rapeseed protein while achieving high solubility (≥88%) without the need to separate fractions, thereby maintaining manufacturing precision and protein quality.
3Ease of manufacture
If conventional extraction methods are used, then processing is simpler, but solubility across pH range is reduced
Solution Approach 1:
The patent introduces a buffered aqueous solution (pH 7.0) as an intermediary extraction medium that maintains optimal pH conditions for the solubility of both cruciferins and napins. This buffer acts as a mediator that preserves protein solubility throughout the extraction and washing processes, enabling the final protein isolate to maintain high solubility (≥88%) across a broad pH range (3-10) while keeping the processing relatively simple without requiring complex fractionation steps.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process achieves a rapeseed protein isolate with solubility of at least 88% over a pH range of 3-10, maintaining high protein content and quality, and preserving the functionality of both cruciferins and napins, suitable for human food consumption without the need for additional fractionation.
Implementation Method 1
mixing cold-pressed rapeseed oil meal with an aqueous liquid at a temperature of from 45 to 65°C
Implementation Method 2
adjusting the pH of the decreamed aqueous liquid obtained in step iii) to a value between pH 6.0 and pH 8.0 by adding acid or base, and mixing with a precipitant to obtain a precipitate; wherein said precipitant comprises a divalent ion salt of magnesium, zinc, iron or calcium
Implementation Method 3
separation of the aqueous liquid from the mixture obtained in step i)
Data Source
Figure 1
AI summary
The present invention is directed to a process for making a soluble native rapeseed protein isolate and the soluble native rapeseed protein isolate obtained by the process.