Recombinant Bacillus Subtilis Fermentation-Isomerization for Psicose Yield
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Solution Overview
Problem
Current fermentation methods for producing psicose have low enzyme activity, leading to low yield and high production costs, making them unsuitable for industrial-scale production.
Innovation Solution
A method involving the fermentation and isomerization of Bacillus subtilis, specifically using recombinant Bacillus subtilis 1A751-DPE, with controlled aeration and rotation speeds, and a tailored fermentation medium, to enhance D-psicose-3-epimerase activity and improve psicose conversion rates.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional fermentation methods are used for psicose production, then the process can be implemented, but the enzyme activity is low resulting in low yield and high production cost
Solution Approach 1:
The patent optimizes fermentation parameters including aeration ratio (0.1-2 V/V·min), rotation speed (200-1000 rpm), temperature (30-37°C), and pH (6-8) to maximize enzyme activity. The fermentation medium composition is also optimized with specific ranges of bean cake powder (10-40 g/L), bran (20-50 g/L), corn flour (30-60 g/L), and minerals to enhance D-psicose-3-epimerase expression and activity, thereby increasing psicose yield while controlling production costs
2Productivity
If conventional fermentation methods are used for psicose production, then the process can be implemented, but the operation is complex
Solution Approach 1:
The patent combines the fermentation process with in-situ enzyme extraction and catalytic reaction steps. The fermented broth is directly used after simple filtration to separate thalli, eliminating the need for separate enzyme extraction and purification steps. This integrated approach simplifies the overall operation while maintaining high psicose yield through the active D-psicose-3-epimerase enzyme
3Reliability
If conventional fermentation methods are used for psicose production, then the process can be implemented, but the enzyme activity is low making it unsuitable for industrial production
Solution Approach 1:
The patent systematically optimizes fermentation parameters including aeration ratio (0.1-2 V/V·min), rotation speed (200-1000 rpm), temperature (30-37°C), and pH (6-8) to maximize enzyme activity. The fermentation medium is optimized with specific compositions of carbon sources, nitrogen sources, and minerals to enhance D-psicose-3-epimerase expression, achieving enzyme activity levels suitable for industrial-scale psicose production
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves a psicose conversion rate of up to 31.4%, simplifies operations, reduces production costs, and uses readily available and inexpensive raw materials, making it suitable for industrial applications while ensuring food safety.
Implementation Method 1
D-psicose-3-epimerase of the recombinant Bacillus subtilis 1A751-DPE was favorably improved
Implementation Method 2
Inoculating recombinant Bacillus subtilis 1A751-DPE seed liquid for expressing D-psicose 3-epimerase into a fermentation culture medium for fermentation culture
Data Source
AI summary
The present invention relates to the technical field of fermentation, and provides a method for producing psicose by fermentation and isomerization of Bacillus subtilis. According to the method of the present invention, fermentation parameters are strictly controlled, and catalytic reaction is performed by using recombinant Bacillus subtilis obtained by fermentation as enzyme liquid, so that the conversion rate of psicose in a system is improved, the problems of low enzyme catalytic reaction efficiency and low yield are solved, and the method is simple to operate and environment-friendly.