Recombinant Ovomucoid Compositions for Clear, Neutral Protein Fortification
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Solution Overview
Problem
There is a need for sustainable, non-animal-based sources of proteins to meet the growing global demand for protein intake, particularly for athletes and bodybuilders, while maintaining sensory qualities and solubility comparable to animal-derived proteins.
Innovation Solution
A recombinant ovomucoid protein (rOVD) with specific glycosylation patterns, devoid of N-linked mannosylation, is used to enhance protein fortification in consumable compositions, providing high solubility, clarity, and sensory neutrality, and is produced using microbial hosts like Pichia pastoris.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If animal-based protein sources are used to meet protein demand, then protein intake requirements are satisfied, but sustainability concerns arise and animal resource consumption increases
Solution Approach 1:
The patent creates a recombinant ovomucoid protein that copies the functional properties of native egg-derived ovomucoid. By producing this protein through microbial fermentation (Pichia pastoris), the invention provides a sustainable alternative to animal-based proteins while maintaining the desired protein content and functional characteristics.
2Object-affected harmful factors
If plant-based protein sources are used as alternatives, then sustainability is improved, but sensory qualities and solubility may be compromised
Solution Approach 1:
The patent employs specific glycosylation engineering to modify the rOVD protein parameters. By controlling N-linked glycosylation patterns (devoid of mannosylation) and using specific microbial hosts, the protein achieves improved solubility and clarity while maintaining sensory neutrality, thus meeting both sustainability and quality requirements.
3Reliability
If recombinant proteins with modified glycosylation are produced, then solubility and clarity are improved, but production process complexity increases
Solution Approach 1:
The patent uses a microbial host (Pichia pastoris) as an intermediary system to produce the recombinant ovomucoid protein with controlled glycosylation. This intermediary approach allows for simplified production compared to traditional animal-derived sources, while achieving the desired solubility and clarity through genetic engineering of the expression system.
Data Source
Figure 1A~1B
Figure 1C~1D
Figure 2~3
AI summary
Provided herein are compositions with enhanced protein content, proteins with high solubility, protein combinations and methods for the preparation thereof.