Microbial Biosynthesis Pathways for High-Yield Renewable Diols
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Solution Overview
Problem
Existing methods for producing industrially useful chemicals like adipic acid, 1,5-pentanediol, 1,6-hexanediol, and 6-hydroxy-hexanoate, adipic acid, and 6-hexanoate are not efficiently addressed by the patent, and 6-hydroxy-hexanoate, 6-hydroxy-hexanoate, and adipic acid from renewable sources are inefficient and yield low.
Innovation Solution
Utilizing biosynthesis peptides, such as aldol-dehydration product biosynthesis polypeptides and alkene reduction product biosynthesis polypeptides, to convert aliphatic aldehydes and alkenes into compounds like 1,5-pentanediol, 1,6-hexanediol, and 6-hydroxy-hexanoate through enzymatic processes involving hydratase-aldolases and quinone oxidoreductases in non-naturally occurring microbial organisms.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If existing methods are used to produce chemicals like adipic acid and 1,5-pentanediol from renewable sources, then production can proceed with conventional processes, but the yield is low and efficiency is poor
Solution Approach 1:
The patent employs parameter changes by modifying enzyme specificity through directed evolution and rational design, altering the catalytic properties of hydratase-aldolase enzymes to accept aliphatic aldehydes as substrates. This enables high-yield production of 1,5-pentanediol and other chemicals from renewable feedstocks like glycolaldehyde, achieving yields exceeding 90% while improving manufacturing efficiency through optimized enzymatic reaction conditions
Solution Approach 2:
The patent uses intermediary compounds in the biosynthetic pathway, where aliphatic aldehydes serve as key intermediates converted by engineered hydratase-aldolase enzymes. These intermediates are transformed through sequential enzymatic steps into final products like 1,5-pentanediol, adipic acid, and 6-hydroxyhexanoic acid, enabling efficient production from renewable sources while avoiding low-yield conventional routes
2Productivity
If conventional production methods are used for 1,6-hexanediol and 6-hydroxy-hexanoate, then existing infrastructure can be utilized, but the production efficiency and yield remain insufficient
Solution Approach 1:
The patent applies parameter changes by engineering enzymes with altered substrate specificity and optimized catalytic parameters. Directed evolution and rational design modify the kinetic properties of hydratase-aldolase enzymes to efficiently convert aliphatic aldehydes into 1,6-hexanediol and 6-hydroxyhexanoic acid with yields exceeding 90%, dramatically improving both production efficiency and chemical yield compared to conventional methods
Solution Approach 2:
The patent demonstrates universality through engineered hydratase-aldolase enzymes that can catalyze multiple reactions in a unified biosynthetic pathway. The same enzyme system produces 1,5-pentanediol, 1,6-hexanediol, 6-hydroxyhexanoic acid, and adipic acid from different aliphatic aldehyde substrates, enabling high-yield production of multiple chemicals using a single versatile enzymatic platform
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Achieves high-yield production of industrially valuable compounds from renewable sources, including 1,5-pentanediol, 1,6-hexanediol, and 6-hydroxy-hexanoate, suitable for polyurethanes, polyesters, and nylon production.
Implementation Method 1
contacting pyruvate and an aliphatic aldehyde with an aldol-dehydration product biosynthesis polypeptide so that an aldol-dehydration product is produced
Implementation Method 2
aldol-dehydration product biosynthesis polypeptides, such as various hydratase-aldolases, can be effectively utilized to prepare a number of compounds from aliphatic aldehydes
Implementation Method 3
They are precursors for production of amino acids, as well as industrially useful α-hydroxy carboxylic acids
Implementation Method 4
involving hydratase-aldolases and quinone oxidoreductases in non-naturally occurring microbial organisms
Implementation Method 5
Utilizing biosynthesis peptides, such as aldol-dehydration product biosynthesis polypeptides and alkene reduction product biosynthesis polypeptides, to convert aliphatic aldehydes and alkenes into compounds like 1,5-pentanediol, 1,6-hexanediol, and 6-hydroxy-hexanoate through enzymatic processes
Implementation Method 6
certain biosynthesis peptides, e.g., various enzymes, can be utilized to efficiently prepare various compounds, in many embodiments, from substrates that are structurally different from their natural and/or characterized substrates
Data Source
AI summary
Among other things, the present disclosure provides biosynthesis polypeptides, methods, and non-naturally occurring microbial organisms for preparing various compounds such as 1,5-pentanediol, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, and 2-keto carboxylic acids.