Repressor-Inactivated Trichoderma Reesei Strain for Cellulase Production
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Solution Overview
Problem
Existing Trichoderma reesei strains have limitations in protein production capabilities, and there is a need to enhance their protein-producing abilities beyond current methods that focus on transcription factor regulation.
Innovation Solution
Introduce mutations that eliminate or reduce the function of specific polypeptides with amino acid sequences represented by SEQ ID NOs: 4, 5, and 6, such as frameshift mutations or deletions, to enhance protein production in Trichoderma reesei strains.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If transcription factor regulation methods are used to enhance protein production, then cellulase production amount is improved, but the protein-producing ability is limited and cannot be further enhanced
Solution Approach 1:
Instead of activating protein production through transcription factors, the invention inactivates repressor proteins (Polypeptide A, B, or C) that suppress protein production. This inverse approach eliminates the braking mechanism rather than trying to accelerate the system, resulting in enhanced protein-producing ability that overcomes the limitations of traditional activation methods
2Quantity of substance
If conventional cultivation methods are used, then basic protein production is maintained, but protein production capabilities cannot be enhanced beyond existing levels
Solution Approach 1:
The invention changes the genetic parameters of the Trichoderma reesei strain by introducing mutations that inactivate repressor proteins. This fundamental parameter change in the organism's genetic makeup enables enhanced protein production capabilities and higher protein concentrations that cannot be achieved through conventional cultivation parameter adjustments alone
Data Source
AI summary
A mutant strain of Trichoderma reesei, the mutant strain having a mutation that eliminates or reduces a function of a polypeptide consisting of the amino acid sequence represented by any of SEQ ID Nos: 4 to 6. A method produces a cellulast, the method including a step of cultivating the mutant strain of Trichoderma reesei, the mutant strain having a mutation that eliminates or reduces a function of a polypeptide consisting of the amino acid sequence represented by any of SEQ ID Nos: 4 to 6.