Resin Analysis Substrate Nanoparticle Signal Extraction
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Solution Overview
Problem
Conventional analysis methods and devices struggle to accurately distinguish between noise signals and nanoparticle detection signals, leading to decreased accuracy in quantitatively analyzing small amounts of detection target substances due to increased noise influence, especially when nanoparticles binding to the reaction region are in low quantities.
Innovation Solution
The method involves using an analysis substrate made of resin material with a reaction region that is irradiated with laser light, extracting substrate and nanoparticle detection signals by comparing light reception levels from reaction and non-reaction regions, and employing nanoparticles with higher signal levels than the substrate signal level to enhance detection accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional analysis method scans reaction region with laser light to detect nanoparticles, then nanoparticle detection is enabled, but noise signals from residues cannot be distinguished from nanoparticle detection signals, leading to decreased measurement precision
Solution Approach 1:
The analysis substrate is divided into reaction regions and non-reaction regions. The non-reaction region is used to measure substrate signal level, while the reaction region is used to detect nanoparticle signals. This segmentation allows separation of substrate background signal from nanoparticle detection signal, enabling accurate distinction between noise and actual signals.
Solution Approach 2:
The non-reaction region acts as an intermediary to measure and subtract substrate signal level from the reaction region signal. By using the non-reaction region as a reference, the system can eliminate substrate background interference and isolate true nanoparticle detection signals from noise.
2Quantity of substance
If nanoparticles binding to detection target substances are decreased to small amount, then sensitivity to low concentration detection is improved, but influence of noise signals is relatively increased, leading to decreased measurement precision
Solution Approach 1:
The invention extracts and removes substrate signal level from the total signal measured in the reaction region. By subtracting the substrate background signal (measured from non-reaction region) from the reaction region signal, the system isolates only the nanoparticle contribution, enabling accurate detection even when nanoparticle amounts are very small.
Solution Approach 2:
The system changes the signal measurement approach by introducing a reference measurement parameter (substrate signal level from non-reaction region) and using it to adjust the reaction region signal. This parameter change enables normalization and background correction, improving precision at low nanoparticle concentrations.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for more accurate detection and quantitation of nanoparticles by distinguishing nanoparticle detection signals with higher signal levels from noise signals, improving the overall accuracy of the analysis process.
Implementation Method 1
irradiating, with laser light, an analysis substrate made of a resin material and having a reaction region on which detection target substances and nanoparticles of a metal compound for labeling the detection target substances are captured; receiving reflected light from the reaction region to generate a light reception level signal
Data Source
AI summary
An analysis method irradiates, with laser light, an analysis substrate made of a resin material and having a reaction region on which detection target substances and nanoparticles of a metal compound for labeling the detection target substances are captured. The analysis method extracts, as a substrate signal level, a signal level generated when receiving reflected light from the analysis substrate. The analysis method receives reflected light from the reaction region to generate a light reception level signal. The analysis method extracts a nanoparticle detection signal from the light reception level signal of the reflected light from the reaction region, the nanoparticle detection signal having a higher level than the signal level of the reflected light from the analysis substrate. The analysis method detects the nanoparticles in accordance with the extracted nanoparticle detection signal.