Resistant Dextrin Production via Compound Enzyme Treatment
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Solution Overview
Problem
Current methods for producing resistant dextrin from starch via high-temperature acidolysis result in low utilization rates and high production costs, with only about 40% of the product being resistant dextrin, making it difficult to meet the demands of mass consumption.
Innovation Solution
A method involving the use of compound enzymes, specifically starch branching enzyme (SBE) and cyclodextrin glycosyltransferase (CGTase), is applied to pyrodextrin obtained through high-temperature acidolysis, increasing the resistant dextrin content to up to 65.3%, improving the utilization rate and reducing production costs.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If high-temperature acidolysis is used to produce resistant dextrin from starch, then the production process is simple, but the resistant dextrin content is low (only about 40%) and production cost is high
Solution Approach 1:
The patent combines high-temperature acidolysis with compound enzyme treatment (starch branching enzyme and cyclodextrin glycosyltransferase) to create a hybrid process. This merging allows the simple acidolysis step to be enhanced by enzymatic reactions, increasing resistant dextrin content from 40% to 65.3% while maintaining process simplicity
Solution Approach 2:
The patent uses a composite enzymatic system consisting of multiple enzymes (starch branching enzyme and cyclodextrin glycosyltransferase) working together. This composite approach leverages the synergistic effects of different enzymes to achieve higher resistant dextrin content than would be possible with a single enzyme or method alone
2Ease of manufacture
If high-temperature acidolysis is used to produce resistant dextrin from starch, then the production process is straightforward, but the utilization rate of starch is low and production cost is high
Solution Approach 1:
By merging acidolysis with enzymatic treatment, the patent improves starch utilization efficiency. The enzymatic steps convert more starch into resistant dextrin, reducing waste and improving overall utilization rate while keeping the process straightforward
Solution Approach 2:
The patent introduces enzymatic reactions with specific temperature and pH parameters that complement the acidolysis conditions. These parameter changes optimize the conversion of starch to resistant dextrin, improving utilization rate without complicating the overall process
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method significantly increases the content of resistant dextrin in the final product, enhancing its enzyme resistance and physiological benefits while lowering production expenses, thus addressing the limitations of existing methods.
Implementation Method 1
Branching enzyme (BE for short, EC 2.4.1.18) belongs to the glycoside hydrolase family (GH13). The enzyme can be widely used to modify starch and catalyze the formation of α-1,6 glycosidic bonds.
Implementation Method 2
Cyclodextrin glycosyltransferase (EC 2.4.1.19, hereinafter referred to as CGTase) is a multi-functional enzyme which can catalyze four reactions: disproportionation reaction (intermolecular transglycosylation), hydrolysis reaction, cyclization reaction (intramolecular transglycosylation) and coupling reaction (reverse reaction of cyclization).
Implementation Method 3
Resistant dextrins are short chain glucose polymers obtained by high-temperature acidolysis of starch
Implementation Method 4
The resistant dextrin is obtained from a pyrodextrin by using a compound enzyme at a high temperature
Data Source
AI summary
Disclosed is a method for preparing a resistant dextrin product by using compound enzyme treatment. Starch is first subjected to high-temperature acidolysis to obtain pyrodextrin which is used as the substrate for the enzyme treatment. A compound enzyme reaction system including a starch branching enzyme and a CGTase is used for preparing the resistant dextrin product. The starch branching enzyme and the CGTase are added simultaneously or sequentially to treat the pyrodextrin to further increase the content of the resistant component in the product. The content of the resistant component of the enzyme treated product reaches up to 65.3%, a 21.3% increase from that of the pyrodextrin before the enzyme treatment.