Rotatable Sample Holder for Faster Light-Sheet Volume Imaging
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Solution Overview
Problem
Existing light sheet microscopes require multiple volume acquisitions due to misalignment between the sample orientation and the scanning direction, leading to inefficiencies in imaging speed and increased phototoxicity, especially with living samples.
Innovation Solution
A sample positioning device with a rotatable sample holder that aligns the sample with the scanning direction of the light sheet microscope, allowing for optimized imaging speed and reduced phototoxicity by minimizing the number of recordings needed.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If multiple volume acquisitions are performed to image the region of interest, then the imaging completeness is improved, but the imaging speed deteriorates and phototoxicity increases
Solution Approach 1:
The invention introduces a rotatable sample holder that enables dynamic reorientation of the sample during imaging. By rotating the sample to align with the scanning direction, the system can acquire complete volumetric data in fewer scans, thereby improving imaging speed while maintaining completeness. This dynamic adjustment resolves the contradiction by making the sample orientation adaptable rather than fixed.
Solution Approach 2:
The invention changes the orientation parameter of the sample by rotating it around the optical axis. This parameter change allows the sample's region of interest to be realigned with the scanning direction, enabling complete imaging in a single volume acquisition and eliminating the need for multiple stitched volumes, thus improving imaging speed.
2Manufacturing precision
If multiple volume acquisitions are performed to image the region of interest, then the imaging completeness is improved, but the phototoxic stress increases
Solution Approach 1:
The rotatable sample holder enables dynamic reorientation of the sample to optimize its alignment with the scanning direction. This dynamic adjustment allows complete volumetric imaging to be achieved in fewer acquisitions, thereby reducing the cumulative phototoxic stress on living samples while maintaining imaging completeness.
Solution Approach 2:
By changing the sample orientation parameter through rotation, the system can capture the complete region of interest in a single volume acquisition or fewer scans. This parameter change reduces the number of times the sample is exposed to illumination, thereby minimizing phototoxic stress while ensuring complete imaging.
3Device complexity
If the sample orientation is fixed by hardware, then the device complexity is reduced, but the adaptability deteriorates
Solution Approach 1:
The invention adds a rotatable sample holder that introduces dynamic orientation adjustment capability to the otherwise fixed hardware system. This dynamic component allows the sample to be rotated to different angles around the optical axis, providing adaptability for different sample orientations and regions of interest without significantly complicating the overall device architecture.
Solution Approach 2:
The rotatable sample holder adds a rotational degree of freedom (another dimension) to the sample positioning capability. This allows the sample to be oriented in different directions around the optical axis, enhancing adaptability while maintaining relative hardware simplicity through a straightforward rotational mechanism.
Data Source
AI summary
A sample positioning device for a light sheet microscope includes a stage insert configured to be received by a stage of the light sheet microscope. The stage insert includes a rotatable sample holder. The rotatable sample holder includes a sample position configured to receive a sample carrier. The rotatable sample holder is configured to be rotatable around an axis of rotation that is parallel to an optical axis of the light sheet microscope, thereby enabling a rotation of a sample received by the sample carrier in a field of view of the light sheet microscope when the stage insert is received by the stage of the light sheet microscope.