S8 Protease Polypeptide Low-Temperature Wash Performance
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Solution Overview
Problem
Current detergents face challenges in achieving high performance at low temperatures and reducing environmental impact, with a need for proteases that are effective in low-temperature washing conditions while being environmentally friendly.
Innovation Solution
Development of an isolated polypeptide with protease activity, specifically a Serine protease of the S8 family from Beauveria brongniartii, which has high sequence identity to the mature polypeptide of SEQ ID NO: 2, and its use in detergent compositions to enhance wash performance and stability across varying pH conditions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional detergents are used for low-temperature washing, then wash performance deteriorates, but using elevated temperatures improves wash performance while increasing energy consumption and environmental impact
Solution Approach 1:
The patent applies parameter changes by modifying the protease enzyme's molecular structure through site-directed mutagenesis, specifically changing amino acid residues to enhance its catalytic activity and stability at low temperatures. This allows the enzyme to maintain high proteolytic activity below 30°C, resolving the contradiction between wash performance and washing temperature by changing the biochemical parameters of the enzyme itself rather than the physical parameters of the washing process
2Reliability
If protease activity is enhanced for low-temperature performance, then stability across varying pH conditions may deteriorate, but maintaining broad pH stability reduces effectiveness in optimized low-temperature conditions
Solution Approach 1:
The patent applies local quality by making specific localized modifications to the protease molecule at key positions affecting temperature sensitivity and pH responsiveness. Through site-directed mutagenesis of specific amino acid residues in the catalytic domain and substrate binding regions, the enzyme gains enhanced low-temperature activity while maintaining adequate pH stability through targeted rather than global modifications
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The polypeptide demonstrates improved wash performance and stability at low temperatures, effectively removing stains and maintaining proteolytic activity in detergents, thus addressing the need for environmentally friendly and efficient low-temperature washing.
Implementation Method 1
polypeptides having protease activity... wherein the polypeptide has at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2
Data Source
Figure 1
AI summary
The present invention relates to isolated polypeptides having protease activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.