Hydrolases TRIZ Cases

Transgenic plants overexpress plastid-specific lipases to accumulate higher oil concentrations in seeds.

Two site-specific nucleases generate paired indels in duplex DNA, maintaining the open reading frame and enabling precise protein function alteration.

Segmented expression cassettes deliver a class 2 type V RNA-guided DNA endonuclease and self-splicing guide RNA for precise fungal genome modification.

Engineered carboxylic acid reductase enzymes replace hazardous chemical catalysts to produce fatty alcohols at higher titers and yields.

Phylogenetic reconstruction generates ancestral Cas9 variants that relax protospacer adjacent motif constraints and reduce immune responses.

A GlcN6P responsive element dynamically regulates gene expression in Bacillus subtilis to drive N-acetylglucosamine synthesis.

Segmenting recognition from cleavage via guide polynucleotides reduces construction complexity while maintaining reliable targeted genome perturbation in yeast.

Identifying IT1 as the identity gene enables precise control over tuber number, size, and formation time by modulating specific protein functions.

Specific amino acid substitutions at positions 208 and 253 reduce odor-causing hydrolysis activity on textiles without compromising cleaning performance.

Isomerase converts R-enantiomer waste into S-enantiomer substrate, achieving near 100% atom utilization.

Engineered ThermoCas9 nucleases maintain activity across broad temperature ranges to enable precise genome editing in thermophilic organisms.

Enzymatic ligation creates circular guide RNAs that resist exonuclease degradation, improving CRISPR editing efficiency and purity.

Using ionic surfactants during lipase immobilization reduces drying time and preserves enzyme activity compared to non-ionic methods.

Detect circulating autoantibodies reactive to 43 kDa proteins via Western blot, eliminating invasive muscle biopsies.

Magnetic yolk-shell nanomaterials purify and immobilize tagged enzymes in one step, resolving trade-offs between stability and process complexity.

A Cas protein fused to a DNA polymerase generates double-stranded DNA fragments from single-stranded donors.

CRISPR/Cas9 knockout of GM-CSF in CAR-T cells reduces cytokine release syndrome and neurotoxicity without compromising antitumor efficacy.

Engineered heterologous hosts synthesize kavalactones and flavokavains, eliminating the unpleasant taste barrier of natural kava extracts.

A CRISPR-Cas13 system uses guide RNAs and plasmonic waveguides to detect viral nucleic acids.

A method for analyzing nuclease hypersensitive sites using sequence-specific restriction enzymes and adapter ligation in aqueous solution.

Reuse lysogoma residue to reduce phosphorus, calcium, and magnesium levels in degummed oil, eliminating costly storage and disposal of harmful byproducts.

A co-delivery platform using lipid nanoparticles to transport gene editors and donor templates into cells.

A mutant thioesterase hydrolyzes medium-chain fatty acid substrates to produce octanoic acid with high catalytic efficiency.

Spacer units link enzymes to porous supports via amide bonds, preserving catalytic activity while enabling easy separation and recycling.

Engineered Type V MAD nucleases overcome limited versatility by altering PAM preferences to broaden targetable genomic sequences in mammalian cells.

TracrRNA stabilizes Type V CRISPR nucleases against high viscosity and metal chelating effects, enabling accurate detection at 45 to 65 degrees Celsius.

Site-directed mutagenesis creates variant phytases that withstand feed pelleting heat and gastric acidity, reducing phosphorus excretion.

Segmented Cas9 proteins linked by a bridge helix require simultaneous target binding to trigger cleavage, eliminating off-target mutations.

Purge valve recycles NAD+ via oxidase enzymes, resolving co-factor imbalance and boosting acetyl-CoA yield to 66.6%.

Transformed Agrobacterium harboring a foreign GABA transaminase gene alters bacterial metabolism to improve plant infection.

Polyoxyethylene derivatives use degradable linkers to extend polypeptide half-life while restoring pharmacological action.

Monoclonal antibodies target unique feline pancreatic lipase epitopes, resolving low sensitivity issues in existing assays for accurate pancreatitis diagnosis.

Digenome-seq detects off-target mutations by cleaving isolated genomic DNA with programmable nucleases, enabling precise guide RNA selection.

A single adeno-associated virus nucleotide encodes a complete CRISPR interference system.

Site-directed mutagenesis of wild-type hydantoinase improves transformation rates and enantioselectivity for D-tryptophan production.

Engineered protease with specific amino acid substitutions at position 99 maintains amylolytic activity in liquid cleaning formulations.

Isolated S8 protease polypeptides enhance detergent wash performance through targeted amino acid modifications.

Segmented Cas protein modules paired with specific guide RNAs resolve the contradiction between rapid multiplex detection speed and operational simplicity.

Coupling Type I-B and Type II CRISPR/Cas machinery with recombineering resolves low transformation rates, raising editing efficiency to 94%.

Optimized halase gene expression in serum-free suspension CHO cells enables scalable production of recombinant protein.

Anti-CRISPR proteins bind Cas endonucleases to reduce off-target cleavage and improve on-target specificity during plant genome editing.

Engineered synthetic phytase variants maintain catalytic activity across broad pH ranges and elevated temperatures.

Complementary deoxyoligonucleotides guide precise DNA repair, reducing frameshift mutations from error-prone non-homologous end joining.

Engineered Cas12f protein variants expand genomic targeting range by altering amino acid substitutions to recognize new PAM sequences.

WDL0090 inbred line resolves breeding efficiency trade-offs by stabilizing disease resistance and stress tolerance traits through preliminary action.

AAV vectors enable sustained hBChE expression, overcoming plasma extraction limits and immunogenicity risks.

Single-stranded nucleic acid templates overcome low spontaneous recombination rates to achieve high-frequency targeted genome modifications.