Yolk-Shell Nanomaterial Enzyme Catalyst for Organophosphorus Degradation
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Solution Overview
Problem
Existing methods for degrading organophosphorus pesticides using biotechnology face challenges such as enzyme instability, non-recyclability, and complex purification processes, leading to incomplete degradation and high costs.
Innovation Solution
A method involving the direct addition of a composite yolk-shell-structured nanomaterial to a crude enzyme solution with an affinity tag to create an organophosphorus degrading enzyme-based multifunctional catalyst, which simplifies the purification process and enhances enzyme stability and recyclability, allowing for effective degradation of organophosphorus pesticides.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional immobilization methods are used to improve enzyme stability and recyclability, then enzyme stability is improved, but the purification process becomes complex
Solution Approach 1:
The patent combines the purification function and immobilization function into a single step by using magnetic nanoparticles functionalized with affinity ligands (such as histidine tags). The enzyme is purified and immobilized simultaneously on the magnetic nanoparticle surface, eliminating the need for separate purification steps while maintaining high enzyme stability and recyclability.
Solution Approach 2:
The patent introduces magnetic nanoparticles with affinity ligands as an intermediary between the enzyme and the purification/immobilization process. These nanoparticles act as a mediator that selectively binds to the enzyme through affinity interactions (e.g., metal-ion affinity with histidine tags), enabling both purification and immobilization in one step while allowing easy separation using magnetic fields.
2Productivity
If enzyme purification is performed to improve degradation efficiency, then degradation completeness is improved, but the process cost increases
Solution Approach 1:
The patent merges purification and immobilization into a single operation using magnetic affinity nanoparticles. This eliminates the need for expensive and time-consuming conventional purification steps while maintaining high enzyme activity and degradation efficiency. The magnetic nanoparticles provide both purification and catalytic functions, reducing overall process costs.
Solution Approach 2:
The enzyme is engineered with self-affinity tags (such as histidine tags) that enable it to automatically bind to the magnetic nanoparticles during the purification/immobilization process. This self-service mechanism eliminates the need for complex external purification procedures, reducing both time and cost while maintaining high degradation efficiency.
3Manufacturing precision
If multiple purification steps are used to improve enzyme purity, then enzyme purity is improved, but the time consumption increases
Solution Approach 1:
The patent combines multiple purification steps into a single magnetic affinity-based purification and immobilization step. The magnetic nanoparticles with specific affinity ligands selectively bind to the target enzyme in one operation, achieving high purity without requiring multiple sequential purification steps, thereby dramatically reducing time consumption.
Solution Approach 2:
The enzyme is pre-modified with affinity tags (such as histidine tags) during gene reconstruction, preparing it in advance for rapid magnetic affinity purification. This preliminary action enables the enzyme to be quickly and efficiently purified and immobilized in a single step, achieving high purity without time-consuming multiple purification steps.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method results in a stable and cost-effective organophosphorus degrading enzyme-based multifunctional catalyst that can completely degrade organophosphorus pesticides into non-toxic products, offering a wide range of applications and improved enzyme utilization rates.
Implementation Method 1
the composite yolk-shell-structured nanomaterial which is rich in transition metal ions in itself is used to further purify and immobilize the organophosphorus degrading enzyme with an affinity tag
Implementation Method 2
subjecting the mixture to a separation, to obtain the organophosphorus degrading enzyme based multifunctional catalyst
Implementation Method 3
subjecting the mixture to a separation is performed by a centrifugation or filtration
Data Source
AI summary
The present disclosure provides a method for preparing an organophosphorus degrading enzyme based multifunctional catalyst and an organophosphorus degrading enzyme based multifunctional catalyst and use thereof. In the present disclosure, the preparation method includes: directly adding a composite yolk-shell-structured nanomaterial into a crude enzyme solution of organophosphorus degrading enzyme with an affinity tag, and mixing, to obtain a mixture, and then subjecting the mixture to a separation, to obtain an organophosphorus degrading enzyme based multifunctional catalyst. According to the present disclosure, the method for preparing an organophosphorus degrading enzyme based multifunctional catalyst is simple in operation, and has a low cost; the multifunctional catalyst prepared by the same has low requirement for the purity of enzyme, support of which could be directionally binded with enzyme, and could be used for detecting an organophosphorus pesticide, and also for a cascade degradation of an organophosphorus pesticide. The final product p-aminophenol has important application value.