Pseudomonas Mendocina A4 for Salt-Tolerant Aerobic Nutrient Removal
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Solution Overview
Problem
Current biological denitrification and phosphorus removal technologies face challenges in simultaneously achieving heterotrophic nitrification, aerobic denitrification, and phosphorus removal under varying environmental conditions, particularly in high-salinity aquaculture environments, leading to eutrophication and operational inefficiencies.
Innovation Solution
A salt-tolerant heterotrophic nitrification aerobic denitrification phosphorus removal bacterial strain, Pseudomonas mendocina A4, capable of degrading NH4+—N, NO3−—N, NO2−—N, and PO43−—P under salinity conditions, which can synchronize nitrification and denitrification in the same aerobic reactor.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional biological denitrification techniques (autotrophic nitrification and heterotrophic denitrification) are used, then nitrogen removal is achieved, but the process requires independent stages and different environmental conditions, increasing system complexity
Solution Approach 1:
The patent combines autotrophic nitrification and heterotrophic denitrification functions into a single bacterial strain (Pseudomonas mendocina A4) that can perform both processes simultaneously. This merging eliminates the need for separate treatment stages and different environmental conditions, reducing system complexity while maintaining nitrogen removal efficiency
Solution Approach 2:
The bacterial strain Pseudomonas mendocina A4 possesses multiple functions: it can perform both nitrification and denitrification, and also remove phosphorus. This multi-functionality allows a single organism to handle multiple pollution removal tasks that traditionally required separate biological processes
2Productivity
If traditional phosphorus removal method using polyphosphate bacteria is applied, then phosphorus removal is achieved, but it requires different environmental conditions (aerobic-anaerobic) from denitrification, creating operational conflicts
Solution Approach 1:
The strain Pseudomonas mendocina A4 can simultaneously perform phosphorus removal and nitrogen removal (nitrification and denitrification) under the same aerobic environmental conditions. This eliminates the need for alternating aerobic-anaerobic cycles required by traditional polyphosphate bacteria, allowing all three functions to operate concurrently without environmental condition conflicts
3Productivity
If previous heterotrophic nitrification aerobic denitrification strains are used, then nitrogen removal under aerobic conditions is achieved, but they lack salt tolerance and phosphorus removal capability, limiting practical applicability
Solution Approach 1:
The strain Pseudomonas mendocina A4 integrates three previously separate capabilities into one organism: heterotrophic nitrification, aerobic denitrification, and phosphorus removal. Additionally, it possesses salt tolerance, making it suitable for aquaculture environments. This multi-functionality resolves the limitation of previous strains that lacked either phosphorus removal capability or salt tolerance
4Productivity
If high-salinity aquaculture environments are treated with conventional methods, then treatment is achieved, but eutrophication occurs due to inability to simultaneously remove nitrogen and phosphorus, leading to operational inefficiencies
Solution Approach 1:
The strain Pseudomonas mendocina A4 can simultaneously remove both nitrogen (through nitrification and denitrification) and phosphorus (through biosorption) under aerobic conditions in high-salinity environments. This dual nutrient removal capability prevents eutrophication by eliminating both limiting nutrients that would otherwise promote algal blooms, addressing the harmful effect of conventional single-nutrient removal methods
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Pseudomonas mendocina A4 achieves efficient nitrogen and phosphorus removal with degradation efficiencies up to 99.58%, 99.99%, and 99.83%, respectively, while tolerating high salinity and organic carbon concentrations, reducing equipment footprint and construction costs, and improving treatment efficiency.
Implementation Method 1
the strain has the function of heterotrophic nitrification and aerobic denitrification
Implementation Method 2
the strain has the function of heterotrophic nitrification and aerobic denitrification
Implementation Method 3
a salt-tolerant heterotrophic nitrification aerobic denitrification phosphorus removal bacterial strain
Data Source
AI summary
The application relates to the field of microbiology and presents a salt-tolerant heterotrophic nitrification aerobic denitrifying and phosphorus removal bacterial strain and its application. The strain is Pseudomonas mendocina A4, deposited on Nov. 4, 2022, at the Guangdong Microbial Culture Collection Center, located at Building 59, 5th Floor, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province, with the deposit number GDMCC No: 62944. The Pseudomonas mendocina A4 strain provided by the present application possesses both heterotrophic nitrification and aerobic denitrification functions and can be applied in the field of wastewater treatment. It has a strong tolerance to high concentrations of organic carbon while utilizing various organic carbon sources in wastewater and exhibits excellent organic carbon removal capabilities in water. This strain is particularly suitable for treating nitrogen-containing wastewater with a high C/N ratio.