Human sCD14-ST Whole Blood Analysis Method
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Solution Overview
Problem
Existing methods for analyzing human sCD14-ST in whole blood samples are prone to inaccurate measurements due to fluctuations in measured values over time, especially when samples are stored or handled improperly, leading to potential misdiagnosis of sepsis.
Innovation Solution
A method for analyzing human sCD14-ST within 6 hours of whole blood sample collection using specific antibodies and biochemical methods, such as chemiluminescent enzyme immunoassay, to ensure accurate measurement without preparing serum or plasma samples, thereby stabilizing the results.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If whole blood samples are stored or allowed to stand at room temperature for extended periods, then the convenience of sample handling is improved, but the measurement precision of human sCD14-ST deteriorates due to decomposition or generation of high molecular weight CD14
Solution Approach 1:
The patent applies preliminary action by establishing a clear time limit (6 hours) for sample analysis before decomposition occurs. This preventive measure ensures that the analysis is performed within the stable period of sCD14-ST, avoiding the need for complex stabilization procedures while maintaining measurement accuracy.
Solution Approach 2:
The patent changes the time parameter from an open-ended storage condition to a specific constrained value (within 6 hours). This parameter change transforms the unstable condition into a controlled condition, ensuring that samples are analyzed within the window where sCD14-ST remains stable and accurate measurements can be obtained.
2Stability of the object's composition
If serum or plasma samples are prepared from whole blood samples, then the stability of human sCD14-ST is improved, but the complexity of the analysis process increases due to additional preparation steps
Solution Approach 1:
The patent extracts the essential requirement for stable measurement (time control) from the complex process of serum/plasma preparation. By removing the need for separation steps and focusing only on the critical time parameter, the method maintains sCD14-ST stability while eliminating unnecessary procedural complexity.
Solution Approach 2:
Instead of following the conventional approach of preparing serum or plasma to ensure stability, the patent inverts the logic by analyzing whole blood directly within a controlled time frame. This inversion eliminates the need for separation procedures while achieving the same stability goal through time-based control.
3Ease of operation
If repeated freezing and thawing of serum samples is performed, then the convenience of sample storage is improved, but the measurement precision of human sCD14-ST deteriorates due to decomposition
Solution Approach 1:
The patent applies preliminary action by analyzing samples within 6 hours of collection, before any freezing or thawing cycles are needed. This preventive approach eliminates the need for repeated storage cycles entirely, avoiding decomposition while maintaining operational convenience through efficient time management.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for precise and stable measurement of human sCD14-ST in whole blood samples, preventing fluctuations and ensuring accurate diagnosis of sepsis by maintaining measured values within a reliable range.
Implementation Method 1
using specific antibodies and biochemical methods, such as chemiluminescent enzyme immunoassay
Implementation Method 2
chemiluminescent enzyme immunoassay
Data Source
AI summary
An analysis method capable of accurately measuring human sCD14-ST when using a whole blood sample is provided. In the analysis method, human sCD14-ST in a whole blood sample is analyzed within 6 hours of the collection of the sample.