Sensor Protein Cell Culture Timing for Stable Chemical Sensing
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing methods for producing odor sensors using lipid bilayer membranes with sensor proteins are inefficient, and there is a need for improved production efficiency and stability of sensor proteins in cells used as chemical sensors.
Innovation Solution
A method for producing cells by culturing cells containing a polynucleotide with a coding sequence for a sensor protein, collecting the cells when the specific activity ratio exceeds 1.2, and optimizing cell concentration and culture period to maintain sensor protein activity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If cells are cultured to produce sensor proteins, then production efficiency is improved, but sensor protein activity is lost
Solution Approach 1:
The patent applies preliminary action by selecting and collecting cells at a specific stage of culture (when specific activity ratio is 1.2 or greater) before complete activity loss occurs. This timing intervention allows production efficiency to be improved while preserving sufficient sensor protein activity for functional use.
Solution Approach 2:
The patent employs feedback by monitoring the specific activity ratio (activity at 1 μM response substance / activity at 0 μM response substance) during cell culture and using this information to determine the optimal collection timing. This feedback mechanism enables maintenance of sensor protein activity while improving production efficiency.
2Productivity
If cells are collected at high concentration, then productivity is improved, but sensor protein activity decreases
Solution Approach 1:
The patent applies dynamics by recognizing that the optimal cell collection concentration is not fixed but varies based on the specific activity ratio. The method dynamically determines collection timing based on real-time activity monitoring, allowing adaptation between cell concentration and activity preservation.
Solution Approach 2:
The patent utilizes parameter changes by monitoring and utilizing the specific activity ratio as a control parameter to determine collection timing. By changing the collection criterion from fixed concentration to variable ratio-based timing, the method optimizes both productivity and reliability.
3Quantity of substance
If culture period is extended, then cell concentration is improved, but sensor protein activity is lost
Solution Approach 1:
The patent applies preliminary action by interrupting the culture process at the optimal timing when specific activity ratio reaches 1.2 or greater, before activity loss becomes complete. This timing intervention preserves sensor protein activity while achieving sufficient cell concentration.
Solution Approach 2:
The patent employs self-service by utilizing the cells' own metabolic activity and response substance binding characteristics to determine optimal collection timing. The cells themselves provide the monitoring mechanism through their inherent sensor protein activity, eliminating the need for external intervention.
Data Source
AI summary
Provided is a method for producing cells for measuring the activity of a sensor protein that can be used as a chemical sensor through culture. The method for producing cells for measuring the activity of a sensor protein includes culturing cells containing a polynucleotide containing a coding sequence for the sensor protein, and collecting the cells when the ratio of the activity value of the sensor protein at a concentration of 1 uM of a response substance to the activity value of the sensor protein at a concentration of 0 µM of the response substance (specific activity) is 1.2 or greater.