Antioxidant Polypeptide from Shark Skin Enzymatic Hydrolysis
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Solution Overview
Problem
Current synthetic antioxidants used in the food industry have carcinogenic effects, and natural antioxidants like A-tocopherol are not effective for food preservation, while terrestrial animal proteins pose safety concerns and are not widely accepted, necessitating a safe and effective natural antioxidant from oceanic sources.
Innovation Solution
An anti-oxidation polypeptide with the amino acid sequence Gly-Ala-Ala-Val-Ala-Leu is extracted from black shark skins using enzymatic hydrolysis and purification methods, specifically using alkali protease and RP-HPLC, to achieve antioxidant activity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If synthetic antioxidants (BHT, TBHQ) are used, then anti-oxidation effect is improved and price is reduced, but carcinogenic effects on human organs occur
Solution Approach 1:
The patent changes the chemical structure parameters of the antioxidant by using specific amino acid sequences (Gly-Ala-Ala-Val-Ala-Leu) derived from black shark skin through enzymatic hydrolysis. This natural polypeptide structure provides antioxidant activity without the carcinogenic properties of synthetic antioxidants like BHT and TBHQ.
Solution Approach 2:
The patent uses a natural, biodegradable polypeptide from fish skin waste as a temporary antioxidant solution that can be safely metabolized by the human body, replacing persistent synthetic antioxidants with potential carcinogenic effects.
2Object-affected harmful factors
If A-tocopherol is used, then natural antioxidant property is improved, but food preservation effectiveness deteriorates
Solution Approach 1:
The patent modifies the antioxidant parameters by using a specific hexapeptide sequence (Gly-Ala-Ala-Val-Ala-Leu) with molecular weight of 500 Da, which demonstrates superior lipid peroxidation inhibition activity compared to A-tocopherol while maintaining natural origin status.
3Quantity of substance
If terrestrial animal proteins are used, then protein source availability is improved, but safety concerns and religious acceptance deteriorate
Solution Approach 1:
The patent extracts collagen protein from black shark skin, an oceanic source, through enzymatic hydrolysis to obtain the antioxidant polypeptide. This extraction from marine sources eliminates concerns about terrestrial animal safety and religious restrictions while maintaining protein availability.
Solution Approach 2:
The patent utilizes black shark skin waste material that has no other high-value use due to its uneven shape and thickness, transforming it into a valuable antioxidant source that serves both waste reduction and health benefit functions.
4Loss of substance
If black shark skins are discarded as waste, then resource waste occurs, but processing cost is reduced
Solution Approach 1:
The patent converts the harmful waste disposal issue into a beneficial resource by using black shark skin waste as the raw material for producing antioxidant polypeptide. The enzymatic hydrolysis process transforms what would be discarded material into a high-value health product.
Solution Approach 2:
The patent recovers valuable collagen protein from black shark skin that would otherwise be discarded, using enzymatic hydrolysis to extract the antioxidant polypeptide and thereby preventing resource waste while creating economic value.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The polypeptide effectively inhibits free radical generation, providing a safe and natural alternative to synthetic antioxidants, enhancing food safety and reducing environmental waste from shark skin processing.
Implementation Method 1
enzymatic hydrolysis of black shark skins, which serve as the raw material, using alkali protease
Implementation Method 2
enzymatic hydrolysis conditions include: 7.0-9.0 pH value, 40-50° C. temperature, 4.0-6.0 h enzymatic hydrolysis time, 2.0-4.0% primer concentration and 9.0-10.0 wt. % enzymes
Implementation Method 3
Separation and purification are completed by using RP-HPLC (reversed-phase high-performance liquid chromatography)
Implementation Method 4
The polypeptide effectively inhibits free radical generation, providing a safe and natural alternative to synthetic antioxidants
Data Source
AI summary
A method of preparing an anti-oxidation polypeptide having an amino acid sequence of SEQ ID NO:1 includes enzymatic hydrolysis of black shark skins, which serve as the raw material, with alkali protease, separation, purification, freezing, and drying to obtain the anti-oxidation polypeptide. Enzymatic hydrolysis conditions include 7.0-9.0 pH value, 40-50° C. temperature, 4.0-6.0 h enzymatic hydrolysis time, 2.0-4.0% primer concentration, and 9.0-10.0 wt % of enzymes.