Silage Fermentation Using L. Plantarum P91 for Tannin Reduction
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Solution Overview
Problem
Silage has a low utilization rate due to a high content of anti-nutritional factors such as tannins and saponins, which affect palatability and digestion in animals.
Innovation Solution
The use of Lactiplantibacillus plantarum P91, isolated from Broussonetia papyrifera silage, which has acid resistance, a high growth rate, and strong carbon source-utilizing ability, is added to silage materials like Caragana korshinskii Kom. and Moringa oleifera Lam. to degrade tannins and saponins, improving fermentation quality and palatability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If woody forages are used as protein feed resources, then protein content and nutrition value are improved, but anti-nutritional factors (tannins and saponins) increase causing reduced palatability and digestion
Solution Approach 1:
The patent utilizes the harmful anti-nutritional factors (tannins and saponins) present in woody forages as substrates for microbial degradation. By introducing specific microorganisms that can metabolize these compounds, the harmful substances are converted into beneficial effects through biological degradation, thereby improving feed quality while maintaining high protein content
Solution Approach 2:
The patent introduces microorganisms as intermediary agents that mediate between the woody forage material and the animal consumer. These microorganisms degrade anti-nutritional factors into less harmful or non-harmful substances, serving as a biological mediator that transforms the toxic feed into safe and nutritious feed
2Quantity of substance
If tannins are present in feed, then protein content is maintained, but palatability and digestion are reduced due to binding with glycoproteins and enzymes
Solution Approach 1:
The patent converts the harmful binding property of tannins with proteins and enzymes into a beneficial degradation process. Microorganisms produce enzymes that break down tannin-protein complexes, releasing the bound proteins and enzymes while degrading tannins into harmless compounds, thereby restoring digestibility while maintaining protein content
3Quantity of substance
If saponins are present in feed, then nutrition value is maintained, but anorexia and reduced nutrient utilization occur
Solution Approach 1:
The patent utilizes saponins as carbon sources for microbial growth and metabolism. The introduced microorganisms degrade saponins through enzymatic hydrolysis and metabolism, converting the harmful bitter-tasting compounds into microbial biomass and harmless metabolic products, thereby eliminating anorexia while preserving nutrition value
4Reliability
If conventional silage fermentation is used, then preservation is achieved, but anti-nutritional factors remain undegraded
Solution Approach 1:
The patent employs multi-functional microorganisms that simultaneously perform conventional silage fermentation (preservation through lactic acid production) and degradation of anti-nutritional factors. These microorganisms exhibit dual functionality: maintaining the preservation benefits of conventional silage while adding the capability to degrade tannins and saponins
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The addition of Lactiplantibacillus plantarum P91 effectively reduces anti-nutritional factors, enhances silage palatability, and improves fermentation activity, offering low-cost, safe, and reliable silage production.
Implementation Method 1
Lactiplantibacillus plantarum P91, which has acid resistance, a high growth rate, and strong carbon source-utilizing ability, is added to silage materials like Caragana korshinskii Kom. and Moringa oleifera Lam. to degrade tannins and saponins
Implementation Method 2
The addition of Lactiplantibacillus plantarum P91 effectively reduces anti-nutritional factors, enhances silage palatability, and improves fermentation activity
Data Source
AI summary
The present disclosure relates to a preparation method of silage, and belongs to the technical field of microbial feeds. The present disclosure is intended to solve the problem that the existing silage has a low utilization rate due to anti-nutritional factors such as tannins and saponins. The present disclosure provides Lactiplantibacillus plantarum P91 with an accession number of CGMCC No. 27567. The Lactiplantibacillus plantarum P91 has acid resistance, a high growth rate, a strong carbon source-utilizing ability, and an excellent comprehensive acid-production ability, and can reduce a pH, well improve the palatability of silage, and reduce anti-nutritional factors in silage. Therefore, the present disclosure can effectively solve the problem that a silage raw material has a high content of anti-nutritional factors such as tannins and saponins, and overcome the adaptability problem of a lactobacillus to a raw material, thereby improving an activity of the lactobacillus and a quality of silage fermentation.