Soluble Bone Marrow Protein Compositions for Enhanced Healing
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Solution Overview
Problem
Current bone grafting techniques face challenges such as infection, poor vascularity, malnutrition, and substantial bone or soft tissue loss, which impede effective osteogenesis, and existing methods for obtaining bioactive factors from tissue sources rely on harsh chemicals, leading to low yields and variability, limiting clinical efficacy.
Innovation Solution
The development of soluble bone marrow protein compositions and scaffolds that include non-recombinant bioactive factors derived from bone marrow mesenchymal stem cells, which are processed to create a dehydrated composition that can be implanted with a scaffold to enhance bone healing, using methods like centrifugation and filtration to isolate and stabilize these factors.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If harsh chemicals are used to extract bioactive factors from tissue sources, then extraction efficiency improves, but product purity and stability deteriorate
Solution Approach 1:
The patent changes the chemical parameters of the extraction process by using mild detergents and buffers instead of harsh chemicals, adjusting pH and ionic strength to optimize extraction while maintaining protein stability and activity
Solution Approach 2:
The patent introduces intermediary substances such as detergents and protective agents that facilitate the extraction of bioactive factors from bone marrow without directly exposing them to harsh conditions, thereby maintaining their stability and function
2Ease of manufacture
If conventional bone grafting methods are used, then surgical procedure is simple, but healing efficacy deteriorates due to infection and poor vascularity
Solution Approach 1:
The patent creates a composite bone graft material that combines bone marrow-derived bioactive factors with a scaffold structure, integrating multiple functional components into a single therapeutic product that maintains surgical simplicity while enhancing healing efficacy
Solution Approach 2:
The patent applies bioactive factors locally to the bone graft site through the scaffold, concentrating therapeutic agents where they are most needed to address specific issues like infection and poor vascularity without requiring complex systemic treatments
3Quantity of substance
If bone marrow is processed without heating, then cell viability is maintained, but protein extraction efficiency deteriorates
Solution Approach 1:
The patent optimizes the temperature parameter during processing, applying controlled heating to enhance protein extraction efficiency while using protective agents and controlled time exposure to prevent excessive thermal damage to cellular components
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The soluble bone marrow protein compositions and scaffolds improve the efficiency and integration of bone grafts, increasing healing efficacy by providing concentrated bioactive factors that can bind to scaffolds, facilitating bone regeneration with reduced immunogenicity and increased stability.
Implementation Method 1
fractionating the bone marrow cell lysate via centrifugation to obtain a soluble bone marrow protein fraction
Implementation Method 2
dehydrating the soluble bone marrow protein fraction to obtain a dehydrated soluble bone marrow protein composition
Implementation Method 3
heating the harvested bone marrow to at least 20° C.
Data Source
AI summary
Provided herein are soluble bioactive factor solutions, grafting scaffolds containing the bioactive factor solutions, and methods of making and using the same.