Soybean Cultivar S100319 Transgenic Trait Introgression

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Solution Overview

Problem

The development of new soybean cultivars is a time-consuming process that requires precise planning and efficient resource use, aiming to combine desirable traits such as high seed yield, disease resistance, drought tolerance, and improved fatty acid profile, while existing methods are limited in efficiently introducing transgenic or mutant traits into soybean cultivars.

Innovation Solution

The introduction of soybean cultivar S100319, which is resistant to glufosinate herbicides and Phytophthora Root Rot, with high yield potential and excellent agronomic characteristics, along with methods for producing transgenic variants by introducing specific genes for traits like herbicide resistance, disease resistance, and modified fatty acid metabolism, using techniques like microprojectile-mediated delivery and Agrobacterium-mediated transformation.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional soybean breeding methods are used to combine desirable traits, then the cultivar achieves high seed yield and disease resistance, but the breeding process takes 6-12 years and requires extensive resources

Engineering Contradiction:
Improvedisease resistanceVSAvoidbreeding process duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-introducing transgenic traits (such as herbicide resistance and disease resistance genes) into the soybean cultivar before traditional breeding begins. This allows the desired traits to be established in advance through genetic transformation, eliminating the need for lengthy traditional breeding cycles to achieve the same result. The cultivar S100319 was developed using this approach, combining multiple desirable traits in a single transformation event or through targeted gene introgression.

Inventive Principle:
Principle #10Preliminary action

2Adaptability or versatility

If traditional breeding methods are used to introduce new traits, then the cultivar achieves improved characteristics, but the process requires multiple generations of crossing and selection

Engineering Contradiction:
Improvetrait combinationVSAvoidbreeding efficiency
Core Design Contradiction:
Adaptability or versatilityVSProductivity

Solution Approach 1:

The patent replaces the mechanical system of traditional physical crossing and selection with genetic engineering techniques. Instead of manually crossing plants and selecting offspring over multiple generations, the invention uses molecular biology methods (such as Agrobacterium-mediated transformation or gene editing) to directly introduce and combine desired traits at the DNA level. This substitution dramatically increases breeding efficiency while achieving the same adaptability and trait combination goals.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Reliability

If extensive crossing and selection is performed to achieve desired traits, then the cultivar achieves superior variety characteristics, but resource consumption and process complexity increase

Engineering Contradiction:
Improveagronomic qualityVSAvoidbreeding process complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent applies parameter changes by modifying the genetic parameters of the soybean cultivar through direct genetic transformation rather than through multiple generations of crossing. By changing the genetic composition at the molecular level (introducing specific genes or gene sequences), the desired agronomic qualities are achieved in a single step or few steps, rather than requiring complex multi-generational breeding programs. This reduces process complexity while maintaining the reliability of the resulting cultivar.

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Soybean cultivar S100319 offers improved yield, disease resistance, and agronomic stability, enabling efficient production of transgenic plants with desired traits, thereby streamlining the breeding process and enhancing soybean quality and functionality.

Implementation Method 1

using techniques like microprojectile-mediated delivery and Agrobacterium-mediated transformation

Methodology Applied
Scientific EffectMicroprojectile-mediated delivery:

Implementation Method 2

using techniques like microprojectile-mediated delivery and Agrobacterium-mediated transformation

Methodology Applied
Scientific EffectAgrobacterium-mediated transformation:

Data Source

PatentUS8507765B2Soybean cultivar S100319
Publication Date: 2013.08.13 M S TECH LLC

AI summary

A soybean cultivar designated S100319 is disclosed. The invention relates to the seeds of soybean cultivar S100319, to the plants of soybean cultivar S100319, to the plant parts of soybean cultivar S100319, and to methods for producing progeny of soybean cultivar S100319. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S100319. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S100319, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S100319 with another soybean cultivar.