Pathogen-Primed Spent Lactic Bacteria Media for Antimicrobial Post-Biotics
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Solution Overview
Problem
Existing methods for enhancing the antimicrobial activity of lactic bacteria are limited in maintaining and increasing the production of functional substances over time, and spent media from lactic bacteria cultures are typically discarded as waste without recognizing their potential antimicrobial properties.
Innovation Solution
Culturing lactic bacteria with pathogen culture derivatives to enhance their antimicrobial activity and isolating the spent media as antimicrobial post-biotic agents, which are then inactivated and optionally lyophilized for use in pharmaceutical and nutraceutical compositions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If lactic bacteria are cultured to produce functional substances, then antimicrobial activity is improved, but spent media is discarded as waste without utilizing its potential antimicrobial properties
Solution Approach 1:
The patent recovers antimicrobial substances from spent media that would otherwise be discarded as waste. The spent media from lactic bacteria cultures is collected and processed to extract active antimicrobial compounds, transforming waste material into a valuable therapeutic product.
Solution Approach 2:
The patent converts the potentially harmful waste product (spent media containing residual bacteria and metabolites) into a beneficial antimicrobial therapy. The spent media, which might be considered waste or even potentially harmful due to residual biological activity, is transformed into a therapeutic agent that fights pathogenic infections.
2Quantity of substance
If pathogen culture derivatives are added to lactic bacteria cultures, then production of functional substances is enhanced, but process complexity increases
Solution Approach 1:
The patent uses pathogen culture derivatives as a preliminary stimulus to prime the lactic bacteria cultures. By pre-exposing the lactic bacteria to pathogen derivatives before or during culture, the system triggers enhanced production of antimicrobial functional substances without requiring complex continuous monitoring or adjustment systems.
Solution Approach 2:
The pathogen culture derivatives act as an intermediary substance that mediates between the lactic bacteria and the desired antimicrobial output. Rather than directly adding complex growth factors or using sophisticated bioreactor systems, the patent uses pathogen derivatives as a simple intermediary trigger that naturally stimulates the bacteria to produce higher quantities of functional substances.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The spent media from lactic bacteria cultures exhibit powerful antimicrobial action, effectively treating and preventing diseases caused by pathogenic agents, demonstrating broad-spectrum efficacy beyond the specific pathogens used in their preparation.
Implementation Method 1
by producing lactic acid, the lactic bacteria in the microbiota reduce the pH, thus creating a restrictive environment that hinders the growth of pathogenic bacteria
Implementation Method 2
The phenomenon can be ascribed to the production of particular functional substances, (among them also the so-called bacteriocins), which are able to act on the one hand indirectly, by modulating the response of the host epithelium, and on the other hand directly, by inhibiting the growth of pathogens
Implementation Method 3
said spent media are inactivated, optionally concentrated and/or lyophilized
Implementation Method 4
EP 0 949 330, in the name of the present Applicant, describes a method for increasing the antimicrobial activity of lactic bacteria by addition of pathogen agents or pathogen culture derivatives to the growth cultures of said lactic bacteria
Data Source
AI summary
Subject-matter of the invention is the use of spent media of lactic bacteria cultures, obtained by addition of pathogen culture derivatives as antimicrobial post-biotic agents, a process for their preparation, and the pharmaceutical and/or nutraceutical compositions comprising said antimicrobial post-biotic agents. Subject-matter of the invention is also the use of pathogen culture derivatives to obtain said antimicrobial post-biotic agents.