Stem Cell Supernatant Fractionation for Cell Activation
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Solution Overview
Problem
The effectiveness of culture supernatants from deciduous tooth pulp stem cells in activating cells is unclear, and they may contain inhibitory components that hinder their efficacy.
Innovation Solution
A cell activator is developed by isolating a fraction from the culture supernatant of deciduous tooth pulp stem cells, excluding components below 3.5 kDa, and focusing on a 50 to 100 kDa range to enhance cell-activating activity while removing potential inhibitors.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If culture supernatant of deciduous tooth pulp stem cells is used directly, then it contains various growth factors and cytokines that may be useful, but it also contains components that act in an inhibitory manner on effective components
Solution Approach 1:
The patent applies extraction by isolating and removing inhibitory components from the culture supernatant. Specifically, the inhibitory fraction is separated through chromatographic methods, and the effective cell-activating fraction is extracted and purified to create a composition free from harmful inhibitors while retaining beneficial growth factors and cytokines.
2Reliability
If the culture supernatant is purified to remove inhibitory components, then cell-activating effects are enhanced, but the complexity of the preparation process increases
Solution Approach 1:
The patent applies segmentation by dividing the complex culture supernatant into distinct fractions based on their functional properties. Chromatographic separation divides the mixture into an inhibitory fraction and a cell-activating fraction, allowing each to be independently characterized and utilized. This segmentation simplifies the understanding of complex interactions and enables targeted purification.
3Reliability
If a specific molecular weight fraction (50 to 100 kDa) is isolated, then cell-activating activity is concentrated, but the quantity of the effective component is reduced
Solution Approach 1:
The patent applies parameter changes by optimizing the molecular weight cutoff parameters during filtration and chromatography steps. By selecting specific molecular weight ranges (50 to 100 kDa), the process concentrates cell-activating activity in the desired fraction. The parameters of filtration membranes and chromatographic conditions are adjusted to maximize both concentration and total quantity of the effective component in the final composition.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The purified fraction exhibits superior cell-activating effects, improving cell functions such as proliferation and metabolism, and is safe and cost-effective to produce.
Implementation Method 1
a fraction isolated from a culture supernatant of a deciduous tooth pulp stem cell, wherein the fraction comprises no component of less than 3.5 kDa
Data Source
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AI summary
Provided is a cell activator comprising a fraction isolated from a culture supernatant of a deciduous tooth pulp stem cell, wherein the fraction comprises no component of less than 3.5 kDa.