Stem Cell Freezing with Wharton's Jelly Conditioned Medium
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Solution Overview
Problem
The low cell numbers in a single umbilical cord blood unit pose a significant challenge for hematopoietic stem cell transplantation, as they fall short of the recommended doses required for successful engraftment, particularly in adults.
Innovation Solution
A method involving the introduction of stem cells into a cell culture medium conditioned with Wharton's Jelly mesenchymal stem cells (WJSCs), followed by slow freezing, which enhances the survival and proliferation of CD34+ cells post-thawing, increasing fold and percentage increases, and reducing apoptotic and dead cell percentages.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If stem cells are stored in conventional freezing media, then storage is achieved, but cell viability and proliferation are reduced due to freezing damage
Solution Approach 1:
The patent uses Wharton's Jelly mesenchymal stem cell conditioned medium as an intermediary substance between the freezing environment and the hematopoietic stem cells. This conditioned medium contains protective factors that mediate the freezing process, reducing direct freezing damage to cells while maintaining storage capability
Solution Approach 2:
The patent changes the chemical composition parameters of the freezing medium by using biologically active conditioned medium instead of conventional chemically-defined media. This parameter change in medium composition provides protective effects during freezing, improving cell viability post-thaw
2Ease of manufacture
If a single umbilical cord blood unit is used, then collection and storage are simplified, but cell numbers are insufficient for successful transplantation
Solution Approach 1:
The patent applies preliminary action by pre-conditioning the culture medium with Wharton's Jelly mesenchymal stem cells before introducing the hematopoietic stem cells for freezing. This preliminary preparation of the medium creates a protective environment that enhances post-thaw proliferation, effectively increasing the functional cell numbers available for transplantation
3Stability of the object's composition
If slow freezing is applied to stem cells, then cell structure is preserved, but freezing time is extended
Solution Approach 1:
The patent changes the thermal and compositional parameters of the freezing process by using conditioned medium with specific protective factors. This parameter change in the freezing environment allows for optimized freezing rates that maintain cell structure while reducing the total time required compared to conventional freezing protocols
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method significantly enhances the fold and percentage increases of CD34+ cells, colony-forming units, and reduces apoptotic and dead cell rates, improving the viability and proliferation of hematopoietic stem cells post-thawing, thereby addressing the limitations of low cell numbers in umbilical cord blood units.
Implementation Method 1
a cell culture medium that has been conditioned with Wharton's Jelly mesenchymal stem cells (WJSCs)
Implementation Method 2
slowly freezing the stem cell culture, thereby freezing the stem cells
Data Source
AI summary
The invention is directed to a method of freezing stem cells comprising introducing the HSCs into a cell culture medium that has been conditioned with Wharton's Jelly mesenchymal stem cells (WJSCs), thereby producing a stem cell culture, and slowly freezing the stem cell culture. In other aspects, the invention is directed to compositions comprising stem cells produced by the methods provided herein. In yet other aspects, the invention is directed to pharmaceutical compositions comprising the stem cells produced by the methods provided herein.