Steroid-Skeleton Surfactant Composition for ApoE Lipid Interference
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Solution Overview
Problem
ApoE protein measurement in blood samples is frequently inhibited by lipids, leading to variable and unreliable results, which complicates the detection of APOE alleles associated with Alzheimer's disease risk and treatment side effects.
Innovation Solution
A composition containing a surfactant with a steroid skeleton is used to suppress lipid interference in ApoE protein measurement, enabling accurate detection even in samples with high lipid content.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If immunoassay is used to measure ApoE protein, then the presence of ApoE4 can be detected, but the measurement is inhibited by lipids in blood samples leading to variable and unreliable results
Solution Approach 1:
The patent introduces a lipid removal step as an intermediary process between sample collection and immunoassay measurement. This intermediary step eliminates the harmful lipid component that causes measurement inhibition, allowing the immunoassay to proceed with accurate and reliable ApoE measurement without interference from blood lipids
Solution Approach 2:
The patent extracts and removes lipids from blood samples before performing ApoE immunoassay measurement. By taking out the harmful lipid component that causes measurement inhibition, the measurement accuracy and reliability are improved while maintaining the ability to detect ApoE4 presence
2Adaptability or versatility
If blood samples with high lipid content are used for ApoE measurement, then the measurement can be performed, but lipid interference causes inhibition and variable results
Solution Approach 1:
A lipid removal step is introduced as an intermediary process that handles samples with varying lipid content. This intermediary step ensures that regardless of the initial lipid content in the blood sample, the measurement process proceeds with consistent accuracy by eliminating lipid interference before the immunoassay
3Device complexity
If ApoE measurement is performed without lipid removal, then the measurement process is simple, but results are inhibited and variable depending on donor lipid content
Solution Approach 1:
The patent extracts and removes lipids from blood samples before performing ApoE immunoassay measurement. By taking out the harmful lipid component that causes measurement inhibition, the measurement accuracy and reliability are improved while maintaining the ability to detect ApoE4 presence
Solution Approach 2:
The lipid removal step is performed as a preliminary action before the immunoassay measurement. By removing lipids in advance, the subsequent measurement process proceeds without interference, ensuring reliable and consistent results across different samples with varying lipid content
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The surfactant effectively prevents lipid inhibition, allowing reliable measurement of ApoE proteins in biological samples, facilitating accurate determination of APOE alleles for Alzheimer's disease risk assessment and treatment monitoring.
Implementation Method 1
a composition for use to suppress inhibition of ApoE protein measurement by a lipid contains a surfactant having a steroid skeleton
Data Source
AI summary
A kit for use to measure an apolipoprotein E (ApoE), comprising: a surfactant having a steroid skeleton; and an ApoE measurement reagent.