Sugar-Based Viral Lysis for High-Yield, Low-Aggregation Manufacturing
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Solution Overview
Problem
Current methods for manufacturing viruses and viral particles face challenges such as the need for environmentally harmful detergents, aggregation of viral particles, and the production of harmful byproducts, particularly at industrial scales.
Innovation Solution
The use of sugar-based detergents, which are biodegradable and environmentally safer, to lyse host cells and release viruses, while maintaining or enhancing the infectivity and yield of the released viruses, and simultaneously inactivating non-target viruses.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional detergents are used to lyse host cells, then virus release efficiency is achieved, but environmental harm and harmful byproduct generation occur
Solution Approach 1:
The patent changes the chemical composition parameter of the detergent from traditional harsh detergents to sugar-based detergents (such as alkyl polyglycosides). This parameter change maintains the lysis effectiveness for virus release while eliminating environmental harm and harmful byproduct generation, as sugar-based detergents are biodegradable and environmentally friendly
Solution Approach 2:
The patent converts the previously harmful traditional detergent into a beneficial sugar-based detergent that not only releases viruses effectively but also provides environmental benefits. The sugar-based detergent becomes a green chemistry solution that turns the harmful lysis process into an environmentally beneficial operation
2Quantity of substance
If host cells are lysed to release viruses, then virus yield is improved, but viral particle aggregation occurs
Solution Approach 1:
The patent changes the chemical parameter of the lysis agent to sugar-based detergent, which has different surface properties compared to traditional detergents. This parameter change prevents viral particle aggregation during the lysis process while maintaining high virus yield, likely due to the milder and more selective action of sugar-based detergents on cell membranes
3Productivity
If industrial scale manufacturing is implemented, then production volume increases, but harmful byproducts are generated
Solution Approach 1:
The patent changes the chemical composition parameter to sugar-based detergents that are biodegradable and environmentally friendly. This parameter change ensures that even at industrial scales, the manufacturing process does not generate harmful byproducts, as sugar-based detergents break down into harmless substances
Solution Approach 2:
The patent transforms the industrial manufacturing process from one that generates harmful byproducts to one that is environmentally beneficial. The sugar-based detergent enables large-scale production while converting potential harm into benefit through biodegradability and environmental safety
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The sugar-based detergents effectively release at least 50% of viruses from host cells with increased infectivity and reduced aggregation, compared to traditional methods, while being environmentally friendly and reducing oxidative damage.
Implementation Method 1
contacting the host cell with a formulation comprising a sugar-based detergent for lysing the host cell
Implementation Method 2
reducing oxidative damage
Data Source
AI summary
Described herein are methods for manufacturing viruses and viral particles. In some aspects, the methods use a sugar-based detergent for lysing a host cell to release the virus or viral particle. Also, described herein is a composition or a pharmaceutical composition comprising the virus or viral particle manufactured by the methods described herein.