Targeted Gluconate Repressor Variant for High-Yield L-Arginine
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Solution Overview
Problem
There is a need for more efficient methods to produce L-arginine with high yield using microorganisms of the genus Corynebacterium, particularly Corynebacterium glutamicum, as existing methods do not adequately address the production requirements.
Innovation Solution
Development of a gluconate repressor variant with specific amino acid substitutions at positions corresponding to SEQ ID NO: 1, such as positions 36, 59, 60, 63, 79, and 92, and the use of a polynucleotide encoding this variant to enhance L-arginine production in Corynebacterium microorganisms.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional methods of increasing enzyme expression or deleting unnecessary genes are used for L-arginine production, then the production process can be simplified, but the yield of L-arginine remains insufficient
Solution Approach 1:
The patent applies parameter changes by modifying specific amino acid residues (positions 36, 59, 60, 63, 79, and/or 92) in the gluconate repressor protein to alter its regulatory function. This changes the protein's parameters (amino acid sequence) to achieve higher L-arginine yield without complex multi-gene modifications, resolving the contradiction between productivity improvement and genetic modification complexity.
2Productivity
If the gluconate repressor protein is modified with specific amino acid substitutions, then L-arginine production yield is improved, but the protein structure becomes more complex
Solution Approach 1:
The patent applies local quality by making targeted modifications at specific positions (36, 59, 60, 63, 79, and/or 92) in the gluconate repressor protein rather than altering the entire protein structure. This localized modification approach improves L-arginine production yield while maintaining overall protein structure simplicity, resolving the contradiction between productivity and structural complexity.
Data Source
AI summary
Provided are a novel gluconate repressor variant, a polynucleotide comprising the variant of the present disclosure, a microorganism of the genus Corynebacterium comprising the variant or polynucleotide of the present disclosure, and a method of producing L-arginine using the microorganism of the present disclosure.