TerC E294K Membrane Protein Variant for L-Lysine Production
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Solution Overview
Problem
Existing methods for producing L-lysine are not sufficiently efficient, necessitating improvements to enhance production capabilities in microorganisms like Corynebacterium glutamicum.
Innovation Solution
Introduction of a membrane protein TerC variant with an amino acid substitution at position 294 from glutamic acid to lysine, along with a polynucleotide encoding this variant, to enhance L-lysine production in Corynebacterium glutamicum strains.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional methods for producing L-lysine are used, then the production process is simple, but the production efficiency is insufficient
Solution Approach 1:
The patent applies parameter changes by modifying a specific amino acid residue (position 294) from glutamic acid to lysine in the membrane protein TerC. This single amino acid substitution alters the protein's properties to enhance L-lysine production efficiency, resolving the contradiction between production efficiency and modification complexity by making a targeted, minimal change rather than a comprehensive redesign
Solution Approach 2:
The invention implements local quality by focusing the modification on a specific local region of the membrane protein (position 294 in the amino acid sequence) rather than altering the entire protein structure. This localized amino acid substitution at a critical position optimizes L-lysine production while maintaining the overall protein structure and function, thereby improving productivity without requiring complete protein redesign
2Productivity
If the expression of genes encoding enzymes involved in L-lysine biosynthesis is increased, then L-lysine production ability is improved, but the overall production efficiency remains insufficient
Solution Approach 1:
The patent changes the parameter of amino acid composition in the membrane protein TerC by substituting glutamic acid with lysine at position 294. This modification enhances the protein's function in L-lysine biosynthesis, leading to improved production ability while maintaining stable and reliable production performance through the optimized protein structure
Data Source
AI summary
The present disclosure relates to a novel membrane protein TerC variant, a Corynebacterium glutamicum strain comprising the variant, and a method for producing L-lysine using the strain.