Ternary Lactic Acid Bacteria Flora for Biogenic Amine Reduction
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Solution Overview
Problem
In the mass production of Huangjiu, biogenic amines accumulate due to the dominance of amine-producing microorganisms, affecting safety, quality, and increasing wastewater treatment costs, while existing biogenic amine degrading lactic acid bacteria lose efficacy in cyclic processes.
Innovation Solution
A ternary interacting flora of Lactiplantibacillus plantarum L-53, Levilactobacillus brevis L-9, and Latilactobacillus curvatus MG-28 is inoculated into the rice steeping process to inhibit amine-producing microorganisms, enhancing acid production and shortening steeping time.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Object-generated harmful factors
If biogenic amine degrading lactic acid bacteria is inoculated into rice milk and rice steeping water is recycled, then biogenic amine content is reduced and production cost is saved, but during mass production the dominant position of inoculated bacteria is gradually replaced and massive accumulation of biogenic amines occurs
Solution Approach 1:
The patent combines three different lactic acid bacteria strains (Lactiplantibacillus plantarum, Levilactobacillus brevis, and Latilactobacillus curvatus) into a ternary symbiotic system. This combination creates synergistic effects where the bacteria mutually enhance each other's ability to suppress amine-producing microorganisms and maintain dominance in the rice steeping environment, preventing the loss of bacterial dominance observed in single-strain systems.
Solution Approach 2:
The invention uses a composite microbial system consisting of multiple bacterial strains working together. Each strain contributes specific metabolic functions and protective mechanisms, creating a composite biological system that is more stable and effective than individual strains, particularly in maintaining dominance during cyclic mass production processes.
2Loss of substance
If rice steeping water is recycled in mass production, then water resources are saved and treatment costs are reduced, but amine producing microorganisms accumulate and affect safety and quality
Solution Approach 1:
The patent applies preliminary anti-action by introducing the ternary symbiotic lactic acid bacteria system into the rice steeping water before amine-producing microorganisms can accumulate to harmful levels. The bacteria preemptively suppress the growth of amine-producing microorganisms through competitive inhibition and metabolic interference, preventing harm rather than treating it after it occurs.
Solution Approach 2:
The system establishes a feedback mechanism where the ternary bacterial system continuously monitors and responds to the microbial environment in the rice steeping water. The bacteria maintain their dominance by dynamically adjusting their metabolic activities to suppress amine-producing microorganisms, creating a self-regulating system that prevents harmful accumulation during water recycling.
3Productivity
If complex microecological floras colonize rice steeping water, then fermentation process is enhanced, but wastewater becomes smelly and treatment costs increase
Solution Approach 1:
The patent applies local quality by focusing the microbial action specifically on the rice steeping water environment rather than allowing uncontrolled microbial colonization throughout the entire system. The ternary lactic acid bacteria system creates a localized controlled fermentation zone that enhances fermentation efficiency while preventing the spread of odor-causing microorganisms to the wastewater stream.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The ternary flora effectively inhibits biogenic amine growth, reducing amine content by 91.15% and shortening steeping time by 40%-70%, with zero amine growth in cyclic processes, and eliminating unpleasant odors, thus improving wine quality and reducing wastewater discharge.
Implementation Method 1
a produced acid from metabolism provides Saccharomyces with an appropriate acidic environment to guarantee smooth fermentation
Implementation Method 2
biogenic amine degrading lactic acid bacteria is inoculated into rice milk and the rice steeping water is recycled
Data Source
AI summary
The disclosure provides microbial composition and application in reducing biogenic amines, and belongs to the technical field of food processing and manufacturing. The disclosure provides a construction method for an inoculated biogenic amine degrading lactic acid bacteria flora and an application in reducing biogenic amines in cyclic rice steeping and persistently inhibiting invasion of amine producing microorganisms. In the disclosure, a lactic acid bacteria ternary interacting flora that efficiently inhibits invasion of the amine producing microorganisms is constructed. In a rice steeping process in preparation of a brewed product, the lactic acid bacteria ternary interacting flora is inoculated for rice steeping, and a cyclic rice steeping process is improved. The result shows that inoculation of the lactic acid bacteria ternary interacting flora and utilization of the improved rice steeping process may effectively reduce the content of biogenic amines in rice steeping water and completely inhibit invasion of the amine producing microorganisms to realize zero growth of the biogenic amines, thereby creating conditions for the cyclic rice steeping process in mass production in a plant.