Time-Lapse Imaging for Abnormal Syngamy Phenotype Detection
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Solution Overview
Problem
Current methods for selecting viable embryos in IVF are largely subjective and rely on morphological evaluations, which have limited predictive value, leading to unsuccessful treatments and potential multiple gestation pregnancies due to inadequate identification of suitable embryos for transfer.
Innovation Solution
The use of time-lapse imaging to measure specific cellular parameters such as syngamy timing and abnormal syngamy phenotypes, allowing for the selection or deselection of embryos based on their likelihood to reach the blastocyst stage, implant, and be euploid, thereby improving the specificity and sensitivity of embryo selection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional morphological evaluation methods are used to select embryos, then the selection process is simple and quick, but the predictive value for embryo viability is limited and subjective
Solution Approach 1:
The patent replaces subjective morphological evaluation with automated time-lapse imaging technology that objectively measures cellular parameters such as division timing, duration, and morphological changes. This substitution of manual assessment with automated optical monitoring systems resolves the contradiction by providing precise, quantifiable data without requiring complex manual analysis procedures.
Solution Approach 2:
The invention introduces new measurable parameters (division timing, duration, morphological changes over time) that transform the embryo selection process from static visual assessment to dynamic quantitative measurement. By changing the parameters being measured from simple appearance to temporal and dimensional characteristics, the predictive value increases while the evaluation remains systematically manageable.
2Reliability
If extended culture to blastocyst stage is used to assess embryo quality, then more information about embryo potential is obtained, but the culture period is prolonged and embryo integrity may be disrupted
Solution Approach 1:
The patent applies preliminary action by measuring and analyzing cellular parameters during early embryonic development stages (cleavage stages) before the embryo reaches the blastocyst stage. By conducting assessments earlier in the development process, the method provides reliable predictions of embryo quality without requiring extended culture periods, thus avoiding disruption of embryo integrity while maintaining assessment accuracy.
Solution Approach 2:
The invention uses feedback from continuous time-lapse monitoring to assess embryo quality parameters at multiple time points during development. This ongoing feedback mechanism allows for reliable quality assessment without requiring the embryo to be cultured to full blastocyst stage, as predictive parameters can be identified and measured during earlier cleavage stages.
3Reliability
If multiple embryos are transferred to ensure successful pregnancy, then the likelihood of pregnancy increases, but the risk of multiple gestation pregnancies increases
Solution Approach 1:
The patent replaces subjective judgment about embryo selection with objective, automated analysis of cellular parameters. This substitution enables more precise identification of embryos with high implantation potential, allowing clinicians to confidently transfer fewer embryos while maintaining or improving pregnancy success rates, thereby reducing the risk of multiple gestations.
Solution Approach 2:
The invention introduces an intermediary assessment system (time-lapse imaging and cellular parameter analysis) that provides detailed information about embryo quality and implantation potential. This intermediary evaluation layer enables more informed decision-making about the number of embryos to transfer, balancing pregnancy success rates against the risks of multiple gestation by providing objective data on individual embryo viability.
Data Source
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AI summary
Methods, compositions and kits for determining the developmental potential of one or more embryos are provided. These methods, compositions and kits find use in identifying embryos in vitro that are most useful in treating infertility in humans.