Decellularized Tissue Bioprosthesis Electrophoretic Antigen Removal
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Solution Overview
Problem
Biological heart valve prostheses have poor durability compared to mechanical devices and face challenges with immunological rejection, necessitating the development of durable, non-immunogenic, and viable alternatives that can be used in children and require effective decellularization and antigen extraction processes.
Innovation Solution
A method involving decellularization with sodium dodecyl sulphate (SDS) followed by an improved rinse procedure and electrophoretic treatment to remove electrically charged substances, ensuring the bioprosthesis is substantially free of SDS and antigens, allowing for recellularization and reduced immunogenic response.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If animal tissue is used to produce bioprostheses, then the supply shortage of autologous tissue is overcome, but immunological rejection occurs
Solution Approach 1:
The patent applies extraction by removing cellular components and antigens from animal tissue through decellularization processes. Specific methods include using detergents, enzymes, or chemical treatments to extract immunogenic elements while preserving the extracellular matrix structure, thereby eliminating rejection risks while maintaining tissue availability
Solution Approach 2:
The patent employs parameter changes by modifying the chemical and physical properties of animal tissue through controlled treatment conditions. By adjusting parameters such as pH, temperature, and chemical concentration during decellularization, the tissue is transformed into a non-immunogenic state while preserving its structural integrity and functional properties
2Object-affected harmful factors
If conventional decellularization methods are used, then antigen extraction is achieved, but durability of the bioprosthesis remains poor
Solution Approach 1:
The patent applies parameter changes by optimizing decellularization conditions to preserve structural integrity. By carefully controlling treatment parameters such as detergent concentration, exposure time, and temperature, the method removes antigens while maintaining the mechanical strength and structural framework of the tissue, thereby improving durability
Solution Approach 2:
The patent employs composite materials by creating a hybrid structure that combines the natural extracellular matrix with enhanced structural support elements. This composite approach reinforces the tissue framework during decellularization, preventing collapse and maintaining durability while allowing thorough antigen extraction
3Object-affected harmful factors
If sodium dodecyl sulphate (SDS) is used for decellularization, then antigen removal is effective, but SDS is difficult to remove from the tissue
Solution Approach 1:
The patent applies continuity of useful action by implementing extended and repeated rinsing procedures. The tissue is subjected to continuous flow of rinsing solutions over extended periods, with multiple solution changes, ensuring thorough and complete removal of SDS residues while maintaining the decellularization benefits
Solution Approach 2:
The patent employs an intermediary approach by using intermediate rinsing solutions that facilitate SDS removal. Specific rinsing agents or buffer solutions are used as intermediaries to break down and flush out SDS residues more effectively than water alone, enabling complete removal without compromising tissue structure
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method enhances the durability and immunocompatibility of bioprostheses, enabling effective recellularization and reducing inflammatory responses, thus providing a viable alternative to allogeneic donor tissue with improved mechanical and structural properties.
Implementation Method 1
tissue is decellularized with the ionic detergent sodium dodecyl sulphate (SDS)
Implementation Method 2
subjecting tissue to an electrophoretic field to extract at least one electrically charged substance from the tissue
Data Source
AI summary
The present invention provides an electrophoretic system, apparatus, and method of use thereof for the preparation of a tissue-derived bioprosthesis.