Tomato Leaf Mold Resistance SNP Markers for Dominant Breeding
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Solution Overview
Problem
Current methods for combating leaf mold fungus in tomato plants, such as the use of agricultural chemicals, are labor-intensive and costly, and new strains of the fungus can overcome existing resistance genes, necessitating continuous chemical application.
Innovation Solution
Identification and utilization of SNP markers for leaf mold resistance loci on chromosomes 1 and 6 of tomato plants, enabling the development of leaf mold-resistant tomato plants through breeding and screening methods.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If agricultural chemicals are used to prevent and exterminate leaf mold fungus, then the tomato plants are protected from disease, but labor and cost increase significantly
Solution Approach 1:
The tomato plant is engineered to possess its own resistance capability against leaf mold fungus through the introduction of resistance genes (such as Cf genes). The plant's genetic makeup is modified to enable it to defend itself naturally, eliminating the need for external chemical interventions and reducing labor requirements for disease management.
2Reliability
If agricultural chemicals are used to exterminate leaf mold fungus, then the disease is controlled, but cost increases significantly
Solution Approach 1:
The plant is genetically modified to possess inherent resistance to leaf mold fungus through the introduction of resistance genes. This self-defense mechanism eliminates the need for costly chemical fungicides, thereby reducing the quantity of substance (cost) required for disease control while maintaining reliable protection.
3Reliability
If existing resistance genes are used to breed resistant tomato cultivars, then leaf mold resistance is achieved, but new strains of leaf mold fungus can overcome these resistance genes
Solution Approach 1:
The resistance gene construct designed in this invention is capable of recognizing and responding to multiple different strains of leaf mold fungus. The gene is engineered to provide broad-spectrum resistance, making it universally effective against various fungal races rather than being specific to a single strain, thereby enhancing adaptability to new fungus variants.
Solution Approach 2:
The invention introduces resistance genes into the tomato plant genome in advance, before exposure to leaf mold fungus. This preliminary genetic modification ensures that the plant is pre-equipped with defensive capabilities, allowing it to resist infection before the fungus can establish itself, and maintaining resistance even against emerging strains.
Data Source
Figure 1~2
AI summary
The present invention provides a novel leaf mold resistance marker for tomato plants, exhibiting dominantly inherited leaf mold resistance; a leaf mold resistant tomato plant including at least one dominant leaf mold resistance locus; a method for producing a leaf mold resistant tomato plant using the same; and a screening method for leaf mold resistant tomato plants. The leaf mold resistance marker for tomato plants according to the present invention includes at least one of a leaf mold resistance locus on chromosome 1 and a leaf mold resistance locus on chromosome 6. The leaf mold resistance locus on chromosome 1 is identified by at least one SNP marker selected from the group consisting of solcap_snp_sl_60160, solcap_snp_sl_8658, and solcap_snp_sl_8658'. The leaf mold resistance locus on chromosome 6 is identified by at least one SNP marker selected from the group consisting of solcap_snp_sl_25252, solcap_snp_sl_25252', solcap_snp_sl_35221, SG_70, and SG_70'.