Microbiological Testing/Measurement TRIZ Cases

A single-tube nested NASBA approach detects nucleic acids in saliva without pre-treatment, improving point-of-care speed and sensitivity.

Automated imaging, heating, and chromogenic culture regions speed home infection testing while improving bacterial identification and treatment guidance.

A restriction enzyme and adapter-PCR workflow quantifies site-specific mitochondrial 6 mA levels for age estimation with fewer artifacts.

Targeted polymerase mutations improve reversible terminator incorporation while keeping low misincorporation error for accurate sequencing.

Solid-supported amine-borane converts oxidized methylcytosines to DHU, improving DNA methylation detection while limiting degradation and toxicity.

Targeting ARHGAP5 in ARHGAP35-deficient cancers exploits paralog lethality to suppress proliferation and restore contact inhibition.

Immobilized LAMP primers on a nanopore thin film enable label-free optical pathogen detection without DNA purification or dye labeling.

A 24-biomarker saliva panel improves prostate cancer detection accuracy beyond PSA and helps assess progression, surgery completeness, and therapy response.

Integrated lateral flow and IVTT use capture strands and nucleic acid barcodes to enable low-cost multiplexed point-of-care detection.

A 36-biomarker saliva panel improves neuroendocrine cancer detection and tracks progression, surgery completeness, and therapy response.

Cleavable mass tags amplify IA-LC-MS/MS signals, enabling pg/mL-level multiplexed analyte detection without losing sensitivity.

Universal probes and tailed primers let dPCR detect multiple nucleic acid targets in one assay using two-color radial fluorescence clusters.

Mesoporous magnetic silica nanopores separate miRNA from protein complexes to improve nucleic acid extraction yield and purity.

Multiple EV surface markers are converted into one amplifiable oligonucleotide, improving isolation specificity and reducing non-specific background.

Linked probes join duplex DNA strands so sense and antisense reads expose sequencing errors early, improving base calling at high throughput.

Ferrocene-labeled primers remove the need for separate signal probes, speeding electrochemical nucleic acid test development and manufacture.

Saliva CpG methylation analysis of ZBTB38 and TRIM6-TRIM34 helps distinguish severe RSV from mild or moderate infection.

Temperature-dependent quenching lets FRET cassettes distinguish multiple nucleic acid targets in one fluorescence channel on standard PCR instruments.

A rotating LED wheel and optical waveguides enable compact multiplex luminescence detection with fewer moving parts and stable temperature control.

Using a low-adhesive substrate and sol-state extracellular matrix, this case enables high-throughput 3D primary cancer cell growth while suppressing fibroblasts.

Segmented hybrid capture probes use complementary end pairing to improve short-fragment enrichment, on-target rate, and hybridization speed.

Artificial marker-bearing nucleic acids enable assembly quality checks, GC bias assessment, and absolute microbial quantification.

APC-presented tumor antigens and cytokine-supported culture enrich and expand tumor-reactive T cells for cancer cell therapy.

A nanopore first screens polynucleotide length by free translocation, then rejects unwanted strands or slows selected ones for detailed sequencing.

SNP markers on tomato chromosomes 1 and 6 enable screening and breeding of dominant leaf mold resistance while reducing chemical control.

Identified ABCG1 phosphorylation sites enable site-specific reagents and antibodies for more precise tumor risk, diagnosis, and prognosis.

Incubating samples with healthy lymphocytes concentrates viable lymphotropic viruses for PCR detection below standard EIA and PCR limits.

Concurrent RNA probes target both antiparallel strands to improve nucleic acid enrichment accuracy in complex biological samples.

Combining cell-free plasma RNA biomarkers improves non-invasive trisomy 21 screening accuracy while reducing false positives and invasive follow-up.

Profiles liver macrophage markers in biopsy tissue with spectral imaging to predict fibrosis risk without phenotype changes from cell isolation.

Combining TIL, T-cell receptor signaling, and mutation burden improves prediction of immunotherapy response and hyper-progression risk.

Chemical and thermal denaturant cycles enable solid-phase nucleic acid amplification with lower instrument cost and fewer non-specific artifacts.

RNA-guided Cas9 cleavage removes restriction-site limits in RFLP genotyping and enables targeted mutagenesis in eukaryotic cells.

Different functional-to-nonfunctional capture reagent ratios enable selective genomic sequencing depth, cutting cost and time for cancer profiling.

A surfactant PCR buffer enables multiplex uveitis pathogen detection from tiny anterior chamber samples while avoiding extraction loss and setup errors.

Stable hairpin chimeric primers cut non-specific background, speed amplification, and improve sensitivity in multiplex nucleic acid detection.

SCGB1D2 and PODXL improve pancreatic cancer and IPMN detection where CA 19-9 lacks early-stage sensitivity and specificity.

Isolated exosomes enable targeted methylation sequencing of bodily-fluid DNA for earlier disease diagnosis, prognosis, and monitoring.

Targeted DPO4 mutations help polymerases incorporate bulky nucleotide analogs more efficiently, improving single-molecule sequencing signals.

Dual enzyme layers and mass transport limiting membranes improve in vivo analyte sensitivity and accuracy while reducing oxygen interference.

Chemical chaperones restore collagen IV folding and export in endothelial cells to reduce apoptosis and vascular lesions in CM-AVM.

An 89Zr-oxine cell label extends PET tracking to several days while lowering radiation dose and preserving cell viability and function.

Engineered polymerases and universal bases suppress non-templated nucleotide addition, keeping spacer length stable across multicycle encoding.

Guar gum boosts gold nanocluster fluorescence for low-cost, stable α-glucosidase detection and inhibitor screening without heavy metals.

Barcode primers and bead-based separation enrich defined and unknown DNA regions, improving sensitive detection of rare alterations.

Direct electrical sensing of complementary polynucleotides in a transmembrane pore speeds RNA profiling while avoiding amplification cost and bias.

NGS-based 16S sequencing replaces slow culture and limited qPCR panels to identify microbes at species level within 2 days.

Floating-gate ChemFET pixels cut body-effect nonlinearity and pixel complexity, enabling dense CMOS pH arrays for rapid analyte measurement.

Targeted disruption of selected TFL1 genes shortens flowering time while preserving vegetative control for faster non-GMO breeding.

Spike-in sequences and control oligos validate patient-specific assays faster and at lower cost while preserving dose-response reliability.