Transgenic Plant Mogroside Production Through Biosynthetic Pathway Transfer
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Solution Overview
Problem
The production of mogrosides from plants like Siraitia grosvenorii is limited and expensive due to the limited natural or agricultural production of these plants, and attempts to produce mogrosides in vitro or in microorganisms have not been economically feasible.
Innovation Solution
The development of transgenic plants and organisms that efficiently produce mogroside compounds, including sweet mogrosides like mogroside V, through the expression of specific polynucleotide sequences encoding cytochrome P450, cucurbitadienol synthase, and other related enzymes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If mogrosides are produced from natural plants like Siraitia grosvenorii, then the sweetener can be obtained with desired properties, but the production cost is high and productivity is limited
Solution Approach 1:
The patent creates transgenic plants that copy the mogroside production pathway from Siraitia grosvenorii into alternative plant species. By transferring the biosynthetic genes (cytochrome P450, cucurbitadienol synthase, glycosyltransferases) into host plants, the technology replicates the natural production capability in a more scalable and cost-effective system, resolving the contradiction between obtaining authentic mogrosides and achieving high productivity
Solution Approach 2:
The patent modifies plant genomes by changing genetic parameters - introducing specific polynucleotide sequences that encode enzymes for mogroside synthesis. This genetic parameter change enables the host plant to produce mogrosides at industrial scales, transforming a low-yield natural system into a high-productivity synthetic biology system
2Productivity
If transgenic plants are developed to produce mogrosides, then production cost decreases and productivity increases, but the complexity of the production system increases
Solution Approach 1:
The patent segments the complex mogroside biosynthesis pathway into individual functional modules - separate genes for cytochrome P450, cucurbitadienol synthase, and glycosyltransferases. Each module can be independently engineered and combined in different configurations, allowing the complex production system to be managed through modular assembly rather than monolithic complexity
Solution Approach 2:
The patent creates a universal transgenic platform that can produce mogrosides in multiple different host plant species. The same set of biosynthetic genes can be transferred into various plants (cucumbers, watermelons, tomatoes, etc.), making the system adaptable and reducing overall complexity by using a single genetic toolkit across different platforms
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables the cost-effective and efficient production of mogroside compounds, potentially reducing production costs and increasing the availability of these valuable sweeteners.
Implementation Method 1
a first polynucleotide sequence that encodes a cytochrome P450 polypeptide... a second polynucleotide sequence that encodes a cucurbitadienol synthase polypeptide... a third polynucleotide sequence that encodes a cytochrome P450 polypeptide... a fourth polynucleotide sequence that encodes a uridine phosphorylase dependent glycosyltransferase polypeptide... a fifth polynucleotide sequence that encodes a uridine phosphorylase dependent glycosyltransferase polypeptide... a sixth polynucleotide sequence that encodes a squalene epoxidase polypeptide... a seventh polynucleotide sequence that encodes an epoxy hydrolase polypeptide... or h) an eighth polynucleotide sequence that encodes a truncated 3-hydroxy-3-methylglutaryl-CoA reductase polypeptide
Data Source
AI summary
The present disclosure provides compositions and methods for producing transgenic plants and other organisms that exhibit increased production of mogroside compounds, in particular mogroside V, and the mogroside compounds, plants and plant parts so produced.