Transition Metal Probe for In Vivo CNS Localization
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Solution Overview
Problem
Current methods lack the ability to directly quantify and map the distribution of transition metals in the central nervous system (CNS) of living subjects, relying on post-mortem analysis or indirect measurements, which are not reliable for in vivo detection in specific regions or tissues.
Innovation Solution
A probe is developed comprising a metal chelating portion, a label portion, and a linker, designed to coordinate with transition metals, react with nucleophiles, and be detectable using medical imaging techniques, allowing in vivo measurement and localization of transition metals.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If post-mortem analysis or indirect measurement methods are used, then transition metal quantification can be performed, but the ability to detect transition metals in specific regions or tissues of living subjects is lost
Solution Approach 1:
The patent introduces a probe as an intermediary substance that can detect transition metals in vivo. The probe comprises a metal chelating portion that specifically binds to transition metals, a label portion that can be detected by medical imaging techniques, and a linker that connects them. This intermediary enables the detection of transition metals in living subjects without requiring post-mortem analysis or indirect measurement methods.
Solution Approach 2:
The patent replaces indirect measurement methods and post-mortem analysis with a direct detection system using a probe that can be administered to living subjects. The probe uses medical imaging techniques (such as PET, SPECT, or MRI) to directly visualize and quantify transition metals in specific regions and tissues of the brain and spinal cord, substituting the need for invasive or indirect approaches.
2Reliability
If fluorescence-based detection is used, then transition metals can be detected, but the method becomes unsuitable for certain medical and veterinary applications
Solution Approach 1:
The patent designs the label portion of the probe to be compatible with multiple medical imaging techniques, including PET (positron emission tomography), SPECT (single-photon emission computed tomography), and MRI (magnetic resonance imaging). This multi-functionality allows the same probe to be used across different medical and veterinary imaging platforms, enhancing its versatility and applicability while maintaining detection accuracy.
3Loss of information
If indirect measurement via imaging of associated structures is used, then some information about transition metal dyshomeostasis can be obtained, but direct quantification of transition metals is not achieved
Solution Approach 1:
The probe is segmented into distinct functional portions: a metal chelating portion for specific transition metal binding, a linker for structural connection, and a label portion for detection. This segmentation allows the probe to specifically target and bind to transition metals while maintaining detectability, enabling direct measurement of transition metal concentration and distribution without relying on indirect markers or associated structures.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate, non-invasive in vivo detection and localization of transition metals in the CNS, facilitating diagnosis of neurological conditions and monitoring metal dyshomeostasis without relying on fluorescence, suitable for use in medical and veterinary medicine.
Implementation Method 1
a metal chelating portion configured to coordinate with a transition metal
Implementation Method 2
the linker is configured to react with a nucleophile when the metal chelating portion coordinates with the transition metal
Data Source
AI summary
The present invention is directed to a probe for detecting a transition metal, the probe comprising: a metal chelating portion configured to coordinate with a transition metal; a label portion configured to be detectable; and a linker bound to both the metal chelating portion and the label portion, wherein the linker is configured to react with a nucleophile when the metal chelating portion coordinates with the transition metal, as well in vivo and in vitro methods and uses thereof.