Multi-pass Transmembrane Polypeptide Aromatic Compound Production
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Solution Overview
Problem
Current methods for producing aromatic compounds like gallic acid using microbes are inefficient, and there is a lack of understanding on how multi-pass transmembrane polypeptides impact their productivity.
Innovation Solution
A transformed cell with enhanced expression of a multi-pass transmembrane polypeptide belonging to the MFS family is used, specifically a polypeptide with an amino acid sequence similar to SEQ ID NO: 2 or its variants, to improve the production of aromatic compounds through the shikimic acid pathway.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional microbial methods are used for producing aromatic compounds, then the production process is simple, but the productivity is low
Solution Approach 1:
The patent segments the production system by introducing specific multi-pass transmembrane polypeptides (MFS family) into the microbial cell to enhance aromatic compound productivity. This segmentation allows targeted improvement of transport functions without redesigning the entire production system, resolving the contradiction between simplicity and productivity.
Solution Approach 2:
The patent changes the parameter of polypeptide expression by introducing and overexpressing specific multi-pass transmembrane polypeptides from the MFS family. This parameter change directly enhances the productivity of aromatic compounds while maintaining the fundamental microbial production system, thus resolving the contradiction between productivity improvement and system complexity.
2Productivity
If multi-pass transmembrane polypeptides are introduced to enhance productivity, then the productivity of aromatic compounds is improved, but the understanding of their function is insufficient
Solution Approach 1:
The patent establishes a feedback mechanism by selecting and validating specific multi-pass transmembrane polypeptides from the MFS family that demonstrably enhance aromatic compound productivity. This feedback approach transforms the previously unknown function of these polypeptides into validated knowledge, resolving the contradiction between productivity improvement and functional understanding.
3Adaptability or versatility
If gene recombination techniques are used to produce aromatic compounds, then diverse compounds can be produced, but the production efficiency is low
Solution Approach 1:
The patent applies universality by using multi-pass transmembrane polypeptides from the MFS family that can enhance the productivity of diverse aromatic compounds (gallic acid, 4-hydroxybenzoic acid, 4-aminobenzoic acid, etc.) through a common mechanism. This universal approach resolves the contradiction between producing diverse compounds and maintaining high production efficiency.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly enhances the productivity of aromatic compounds such as gallic acid and protocatechuic acid, achieving efficient production through an environmentally friendly fermentation method.
Implementation Method 1
multi-pass transmembrane polypeptide belonging to the MFS family (major facilitator superfamily) have been reported to include proteins involved in stress resistance and tolerance
Implementation Method 2
it has been desired to produce useful aromatic compounds including gallic acid using microbes from an inexpensive starting material glucose
Implementation Method 3
phosphoenolpyruvic acid formed in the glycolysis system binds to erythrose 4-phosphate supplied from the pentose phosphate pathway, to thereby provide 3-deoxy-D-arabinoheptulosonic acid 7-phosphate (DAHP)
Data Source
AI summary
Provided are a method for producing an aromatic compound or a salt thereof using a transformed cell capable of producing the aromatic compound or the salt thereof, and this transformed cell. The present invention provides a method for producing an aromatic compound or a salt thereof, comprising the step of culturing a transformed cell with enhanced expression of a multi-pass transmembrane polypeptide represented by the following (A) or (B): (A) a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2, and (B) a polypeptide consisting of an amino acid sequence having at least 76% identity to the amino acid sequence represented by SEQ ID NO: 2.